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Antikörper

MERTK Monoclonal Antibody

Produktgrößen
50 ul
£235,00
CSB-MA621519A0M-50UL
100 ul
£392,00
CSB-MA621519A0M-100UL
Über dieses Produkt
SKU:
CSB-MA621519A0M
Zusätzliche Namen:
c MER antibody; c mer proto oncogene tyrosine kinase antibody; c-mer antibody; cMER antibody; cmer protooncogene tyrosine kinase antibody; Eyk antibody; MER antibody; MER receptor tyrosine kinase antibody; MERK antibody; MERPEN antibody; Mertk antibody; MERTK c-mer proto-oncogene tyrosine kinase antibody; MERTK_HUMAN antibody; MGC133349 antibody; nmf12 antibody; Nyk antibody; Proto oncogene tyrosine protein kinase MER antibody; Proto oncogene tyrosine protein kinase MER precursor antibody; Proto-oncogene c-Mer antibody; Receptor tyrosine kinase MerTK antibody; RP38 antibody; STK kinase antibody; Tyrosine-protein kinase Mer antibody
Anwendung:
ELISA, Flow Cytometry, Immunofluorescence, Western Blot
Buffer:
PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.
translate.label.attr.clone:
16D1
Klonalität:
Monoclonal
Host:
Mouse
Immunogen:
Recombinant MERTK protein (21-505AA)
Isotyp:
IgG2b
Physischer Zustand:
Liquid
Aufreinigung:
Protein A Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Monoclonal Antibody
Overlay Peak curve showing 293T transfected cells stained with CSB-MA621519A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106 cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunofluorescence staining of 293T transfected cells with MERTK CSB-MA621519A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Immunofluorescence staining of HepG2 cells with MERTK CSB-MA621519A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Western Blot Positive WB detected in: 1-4 lanes: 293T whole cell lysate transfected with MERTK at 10ug, 5ug, 2.5ug, 1.25ug All lanes: MERTK antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 150-250 KDa Observed band size: 150-250 KDa Exposure time : 5S
Western Blot Positive WB detected in: 1-4 lanes: Recombinant MERTK protein at 20ng, 10ng, 5ng, 2.5ng All lanes: MERTK antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 120 KDa Observed band size: 120-150 KDa Exposure time : 5S