Skip to content

Warenkorb

Der Warenkorb ist leer.

Gesamt (exkl. MwSt.) £0,00
Warenkorb überprüfen & bezahlen
Antikörper

HSPA8 Monoclonal Antibody

Produktgrößen
50 ul
£235,00
CSB-MA010829A0M-50UL
100 ul
£392,00
CSB-MA010829A0M-100UL
Über dieses Produkt
SKU:
CSB-MA010829A0M
Zusätzliche Namen:
Heat shock cognate 71 kDa protein (Heat shock 70 kDa protein 8) (Lipopolysaccharide-associated protein 1) (LAP-1) (LPS-associated protein 1), HSPA8, HSC70 HSP73 HSPA10
Anwendung:
ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
2G8F6
Klonalität:
Monoclonal
Weitere Details:
Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase CHIP
Host:
Mouse
Immunogen:
Recombinant Human Heat shock cognate 71 kDa protein (2-646AA)
Isotyp:
IgG2b
Physischer Zustand:
Liquid
Aufreinigung:
Protein A Purified
Reaktivitäten:
Human, Mouse, Rat
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Monoclonal Antibody
Overlay histogram showing MCF-7 cells stained with CSB-MA010829A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the primary antibody at 1/200 for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b used under the same conditions. Acquisition of >10,000 events was performed.
IHC image of CSB-MA010829A0m diluted at 1:256 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight, and detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-MA010829A0m diluted at 1:256 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight, and detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating HSPA8 in Hela whole cell lysate Lane 1: Mouse control IgG2b instead of CSB-MA010829A0m in Hela whole cell lysate. Lane 2: CSB-MA010829A0m (1.5ul) + Hela whole cell lysate (500ug) Lane 3: Hela whole cell lysate (20ug) For western blotting, the blot was detected with CSB-MA010829A0m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
Western Blot Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Mouse spleen tissue, Rat spleen tissue, Mouse heart tissue, Rat heart tissue All lanes HSPA8 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 10s