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Antikörper

CD48 Monoclonal Antibody

Produktgrößen
50 ul
£235,00
CSB-MA004941A1M-50UL
100 ul
£392,00
CSB-MA004941A1M-100UL
Über dieses Produkt
SKU:
CSB-MA004941A1M
Zusätzliche Namen:
Antigen CD48 antibody; B cell membrane protein antibody; B lymphocyte activation marker BLAST 1 antibody; B-cell activation marker antibody; B-lymphocyte activation marker BLAST-1 antibody; BCM 1 surface antigen antibody; BCM1 antibody; BCM1 surface antigen antibody; BLAST 1 antibody; BLAST antibody; BLAST1 antibody; CD 48 antibody; CD48 antibody; CD48 antigen (B cell membrane protein) antibody; CD48 antigen antibody; CD48 molecule antibody; CD48 protein antibody; CD48_HUMAN antibody; hCD48 antibody; Leucocyte antigen MEM 102 antibody; Leukocyte antigen MEM-102 antibody; mCD48 antibody; MEM 102 antibody; MEM-102 antibody; MEM102 antibody; Signaling lymphocytic activation molecule 2 antibody; SLAM family member 2 antibody; SLAMF 2 antibody; SLAMF2 antibody; TCT.1 antibody
Anwendung:
ELISA, Flow Cytometry, Immunofluorescence, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
26C2C11
Klonalität:
Monoclonal
Host:
Mouse
Immunogen:
Recombinant Human CD48 protein (27-220AA)
Isotyp:
IgG1
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human, Mouse
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Monoclonal Antibody
Overlay Peak curve showing JK cells stained with CSB-MA004941A1m (red line) at 1:200. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106 cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunofluorescence staining of JK cells with CSB-MA004941A1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Western Blot Positive WB detected in: Jurkat whole cell lysate, Raji whole cell lysate, Daudi whole cell lysate, Mouse liver tissue All lanes: CD48 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 28, 20 KDa Observed band size: 43 KDa Exposure time : 5min
Western Blot Positive WB detected in: 20ug Raji whole cell lysate CD48 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 28, 20 KDa Observed band size: 43 KDa Exposure time : 5min