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100 tests
£170,00
PTN-P-101-100-100TESTS
Über dieses Produkt
SKU:
PTN-P-101-100
Weitere Details:
To deliver antibodies into living cell. It can easily introduce antibodies into living cell effcientlly by usuing function of artificial cell penetrating peptide, controlling cytotoxicity. Features Suitable for use with 10% serum-containing media. Suitable for various antibodies(polyclonal, monoclonal). It is also possible to introduce fluorescently labeled antibodies and enzyme-labeled antibodies. Stable at 4 ° C for 6 months. The activity does not decrease even after freezing and thawing (10 times). Cell lines with a proven track record (will be added at any time) Ready-to-use. Easy and quick procedure. Very low cytotoxicity. Specificity: Endotxin level < 0.05 EU / ul Antibody transfectionactivity (relative value)* 80 - 150 % * (BETA) -galactosidase-conjugated goat IgG was transfected to HeLa cells and incubated for 4 hrs. Antibody transfection activity was estimated by (BETA)- galactosidase activity in lysates of the cells. The values represent relative activity (%) to that of Lot N12J15 as standard. References 1) FEBS Journal (2015) 282: 142-152. Effective capture of proteins inside living cells by antibodies indirectly linked to a novel cell-penetrating polymer-modified protein A derivative. Shoko Itakura, Susumu Hama, Hisafumi Ikeda, Naoto Mitsuhashi, Eiji Majima and Kentaro Kogure. 2) Mol Cell Biochem (2015) 404: 25-30. Effects of employment of distinct strategies to capture antibody on antibody delivery into cultured cells. Kana Kuwahara, Kazuki Harada, Ryohei Yamagoshi, Takenori Yamamoto, Yasuo Shinohara., PTN
Versandbedingungen:
Blue Ice
Lagerbedingungen:
2-8[o]C
Hersteller:
Cosmo Bio Ltd
Typ:
Assay Kits: Labelling