Alkaline Phosphatase Staining Kit
Produktgrößen
1 kit
PMC-AK20-1KIT
Über dieses Produkt
- SKU:
- PMC-AK20
- Weitere Details:
- Alkaline Phosphatase Staining Kit is designed for the rapid and sensitive detection of osteoblasts differentiated in culture from bone marrow cells. Bone forming osteoblasts and bone-degrading osteoclasts can be distinguished by the expression, respectively, of alkaline phosphatase and tartrate-resistant acid phosphatase (TRAP). CosmoBio's TRAP staining kit (PMC-AK04F-COS) is ideal for rapid and convenient detection of the latter. Simple staining protocol
- Remove culture medium. Wash each well 3 times with 1mL PBS. Add 500uL of 10% formalin neutral buffer solution to each well and fix for 20 minutes at room temperature. Remove 10% formalin neutral buffer solution and wash each well 3 times with 2mL deionized water. Dissolve 1 vial of Chromogenic Substrate in 5mL Substrate-containing Buffer. Add 400uL Chromogenic Substrate to each well. Incubate at 37°C for 5-20 minutes. Adjust incubation time until stained. Wash with deionized water to stop the reaction.
- A. S. H. De Jong, M. Van Kessel-Van Vark and A. K. Raap. Sensitivity of various visualization methods for peroxidase and alkaline phosphatase activity in immunoenzyme histochemistry. The Histochemical Journal Volume 17, 1119-1130 (1985)
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- 2-8[o]C
- Hersteller:
- Cosmo Bio Ltd
- Typ:
- Assay Kits: Staining & Imaging