QuickTiter™ HBV Core Antigen ELISA Kit
Produktgrößen
96 assays
£1065,00
VPK-150-96ASSAYS
5 x 96 assays
£4219,00
VPK-150-5X96ASSAYS
Über dieses Produkt
- SKU:
- VPK-150
- Anwendung:
- Detection, ELISA
- Weitere Details:
- An anti-HBVcAg monoclonal coating antibody is adsorbed onto a microtiter plate. HBV core antigen present in the sample or standard binds to the antibodies adsorbed on the plate; a FITC-conjugated mouse anti-HBVcAg antibody is added and binds to the antigen captured by the first antibody. Following incubation and wash steps, a HRP-conjugated mouse anti-FITC antibody is added and binds to the FITC conjugated anti-HBVcAg. Following unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of HBV core antigen present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from recombinant HBV core antigen and sample concentration is then determined.
- Format:
- Colorimetric
- Forschungsgebiet:
- Pathogen and Toxin Assays
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- Upon receiving, aliquot and store recombinant HBVcAg Standard at -20ºC and avoid freeze/thaw. Store all other components at 4ºC.
- Hersteller:
- CellBioLabs
- Typ:
- Assay Kits:Assays
- Detection Method:
- Colorimetric