DAG (Diacylglycerol) Assay Kit
Produktgrößen
100 assays
£676,00
MET-5028-100ASSAYS
Über dieses Produkt
- SKU:
- MET-5028
- Weitere Details:
- Our DAG (Diacylglycerol) Assay Kit measures diacylglycerol in cell lysate samples by a coupled enzymatic reaction system. First, kinase is used to phosphorylate DAG in samples, yielding phosphatidic acid. Next, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. The glycerol-3-phosphate product is then oxidized by glycerol-3-phosphate oxidase, producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. DAG levels in unknown samples are determined based on the provided DAG standard curve.
- Format:
- Fluorometric
- Physischer Zustand:
- Cell Lysate
- Forschungsgebiet:
- Metabolism Research
- Empfindlichkeit:
- 4uM DAG
- Versandbedingungen:
- Dry Ice
- Lagerbedingungen:
- Store the entire kit at -80ºC. Avoid multiple freeze/thaws by aliquoting. The Fluorometric Probe is light sensitive and should be maintained in amber tubes.
- Hersteller:
- CellBioLabs
- Typ:
- Assay Kits:Assays
- Detection Method:
- Fluorometric