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Proteine und Peptide

Human Interleukin 9 (IL9), Active Protein

Produktgrößen
50 ug
£934,00
RPU59234-50UG
100 ug
£1351,00
RPU59234-100UG
1 mg
£5642,00
RPU59234-1MG
Über dieses Produkt
SKU:
RPU59234
Zusätzliche Namen:
Homolog Of Mouse T Cell And Mast Cell Growth Factor 40|HP40|P40|P40 Cytokine|P40 T-Cell And Mast Cell Growth Factor|T Cell Growth Factor III|TCGF3|TCGFIII
Anwendung:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Weitere Details:
Human Interleukin 9 (IL9), Active Protein is a purified Prokaryotic Protein.
Formulierung:
Freeze-dried powder
Immunogen:
Interleukin 9 (IL9)
Molekulargewicht:
18kDa
Physischer Zustand:
freeze-dried
Reinheit:
>95%
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles.
Hersteller:
Biomatik
Typ:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
Figure . SDS-PAGE
Figure . Western Blot
Interleukin 9, also known as IL-9, is a pleiotropic cytokine belonging to the group of interleukins. IL-9 secreted by CD4 helper cells that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin-9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins namely STAT1, STAT3 and STAT5 and thus connects this cytokine to various biological processes. Besides, Interleukin 9 Receptor (IL9R) has been identified as an interactor of IL-9, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL-9 and recombinant human IL9R. Briefly, IL-9 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL9R-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL-9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL-9 and IL9R was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of IL-9 with IL9R