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Proteine und Peptide

Human Myostatin (MSTN), Active Protein

Produktgrößen
50 ug
£915,00
RPU59180-50UG
100 ug
£1326,00
RPU59180-100UG
1 mg
£5194,00
RPU59180-1MG
Über dieses Produkt
SKU:
RPU59180
Zusätzliche Namen:
GDF8|Growth Differentiation Factor 8
Anwendung:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Weitere Details:
Human Myostatin (MSTN), Active Protein is a purified Eukaryotic Protein.
Formulierung:
Freeze-dried powder
Immunogen:
Myostatin (MSTN)
Molekulargewicht:
54kDa
Physischer Zustand:
freeze-dried
Reinheit:
>95%
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles.
Hersteller:
Biomatik
Typ:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
Figure . SDS-PAGE
Myostatin (MSTN) also known as growth differentiation factor 8 (GDF-8) is a member of the TGF beta protein family. Myostatin is a secreted growth differentiation factor that produced and released by myocytes. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Besides, Bone Morphogenetic Protein 1 (BMP1) has been identified as an interactor of MSTN, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MSTN and recombinant human BMP1. Briefly, MSTN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to BMP1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-MSTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of MSTN and BMP1 was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of MSTN with BMP1.