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Proteine und Peptide

Human Interferon Alpha (IFNa), Active Protein

Produktgrößen
50 ug
£896,00
RPU59162-50UG
100 ug
£1298,00
RPU59162-100UG
1 mg
£5076,00
RPU59162-1MG
Über dieses Produkt
SKU:
RPU59162
Zusätzliche Namen:
IFL|IFN|IFN-A|IFN-Alpha|IFN-Alpha 1b|IFN, Leukocyte|IFNA1|IFNA13|IFNAP22|Interferon Alpha 1b|Interferon alpha-D|Interferon, Leukocytic|LeIF D
Anwendung:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Weitere Details:
Human Interferon Alpha (IFNa), Active Protein is a purified Prokaryotic Protein.
Formulierung:
Freeze-dried powder
Immunogen:
Interferon Alpha (IFNa)
Molekulargewicht:
48kDa
Physischer Zustand:
freeze-dried
Reinheit:
>90%
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles.
Hersteller:
Biomatik
Typ:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
Figure . SDS-PAGE
Figure . Western Blot; Sample: Recombinant Human, IFNa; Antibody: Rabbit Anti-IFNa Human Ab (PAA033Hu)
Figure . Gene Sequencing (Extract)
Interferon-alpha (IFN-A Alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits. IFN-A Alpha has both anti-viral and immunomodulatory activities on target cells. To test the effect of IFN-A Alpha on cell apoptosis, A549 cells were seeded into 96-well plates at a density of 3,000 cells/well with 1% serum standard DMEM including various concentrations of recombinant human IFN-A Alpha. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2 hours at 37°C. Proliferation of A549 cells after incubation with IFN-A Alpha for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant human IFN-A Alpha for 48h. The result was shown in Figure 2. It was obvious that IFN-A Alpha significantly inhibit cell viability of A549 cells. The ED50 is 3.4 ug/mL.(A) A549 cells cultured in DMEM, stimulated with 10ug/mL IFN-A Alpha for 48h;(B) Unstimulated A549 cells cultured in DMEM for 48h.Figure 1. Inhibition of A549 cells proliferation after stimulated with IFN-A Alpha