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Proteine und Peptide

Human Fibroblast Growth Factor 23 (FGF23), Active Protein

Produktgrößen
50 ug
£810,00
RPU59098-50UG
100 ug
£1237,00
RPU59098-100UG
1 mg
£4543,00
RPU59098-1MG
Über dieses Produkt
SKU:
RPU59098
Zusätzliche Namen:
ADHR|HPDR2|HYPF|Phosphatonin|PHPTC|Tumor-derived hypophosphatemia-inducing factor
Anwendung:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Weitere Details:
Human Fibroblast Growth Factor 23 (FGF23), Active Protein is a purified Prokaryotic Protein.
Formulierung:
Freeze-dried powder
Immunogen:
Fibroblast Growth Factor 23 (FGF23)
Molekulargewicht:
11kDa
Physischer Zustand:
freeze-dried
Reinheit:
>95%
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles.
Hersteller:
Biomatik
Typ:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
Figure . SDS-PAGE
Figure . Western Blot
Figure. The binding activity of FGF23 with FGFR2.Fibroblast growth factor 23 or FGF23 is a member of the fibroblast growth factor (FGF) family which is responsible for phosphate and vitamin D metabolism. The main function of FGF23 seems to be regulation of phosphate concentration in plasma. FGF23 decreases the reabsorption and increases excretion of phosphate and suppress 1-alpha-hydroxylase, reducing its ability to activate vitamin D and subsequently impairing calcium absorption. Besides, Fibroblast Growth Factor Receptor 2 (FGFR2) has been identified as an interactor of FGF23, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF23 and recombinant human FGFR2. Briefly, FGF23 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to FGFR2-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-FGF23 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of FGF23 and FGFR2 was shown in Figure 1, and this effect was in a dose dependent manner.