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Proteine und Peptide

Human Chitinase-3-like Protein 1 (CHI3L1), Active Protein

Produktgrößen
50 ug
£909,00
RPU56345-50UG
100 ug
£1140,00
RPU56345-100UG
1 mg
£5358,00
RPU56345-1MG
Über dieses Produkt
SKU:
RPU56345
Zusätzliche Namen:
39 kDa synovial protein|CHI3-L1|Chitinase 3-Like 1|Chondrex|Chondrocyte Protein YKL40|GP39|YKL40
Anwendung:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Weitere Details:
Human Chitinase-3-like Protein 1 (CHI3L1), Active Protein is a purified Prokaryotic Protein.
Formulierung:
Freeze-dried powder
Immunogen:
Chitinase-3-like Protein 1 (CHI3L1)
Molekulargewicht:
26kDa
Physischer Zustand:
freeze-dried
Reinheit:
>97%
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles.
Hersteller:
Biomatik
Typ:
Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
Catalog Protein.JPG
Figure . SDS-PAGE
Figure . Western Blot
Figure. Cell proliferation of Raji cells after stimulated with GP39.Glycoprotein 39, Cartilage (GP39) also known as Chitinase-3-like protein 1 (CHI3L1) is a secreted glycoprotein that is approximately 40kDa in size that in humans is encoded by the CHI3L1 gene. GP39 plays a role in cancer cell proliferation, survival, invasiveness and in the regulation of cell-matrix interactions. To test the effect of GP39 on cell proliferation, Raji cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 1% serum standard 1640 which contains various concentrations of recombinant human GP39. After incubated for 5 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of Raji cells after incubation with GP39 for 5 days observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant GP39 for 5 days. The result was shown in Figure 2. It was obvious that GP39 significantly increased cell viability of Raji cells.(A) Raji cells cultured in 1640, stimulated with 10ng/mL GP39 for 5 days; (B) Unstimulated Raji cells cultured in 1640 for 5 days.