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Proteine und Peptide

Human Insulin Like Growth Factor 1 (IGF1), Active Protein

Produktgrößen
50 ug
£302,00
RPU56075-50UG
100 ug
£347,00
RPU56075-100UG
1 mg
£781,00
RPU56075-1MG
Über dieses Produkt
SKU:
RPU56075
Zusätzliche Namen:
IBP1|IGF1A|IGFI|Mechano Growth Gactor|MGF|Somatomedin C
Anwendung:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Weitere Details:
Human Insulin Like Growth Factor 1 (IGF1), Active Protein is a purified Prokaryotic Protein.
Formulierung:
Freeze-dried powder
Immunogen:
Insulin Like Growth Factor 1 (IGF1)
Molekulargewicht:
40kda
Physischer Zustand:
freeze-dried
Reinheit:
>95%
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles.
Hersteller:
Biomatik
Typ:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
Figure . SDS-PAGE
Figure . Western Blot
Figure . Gene Sequencing (Extract)
To measure the effect of IGF1 on cell proliferation, breast cancer MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 1% serum standard DMEM prior to the addition of various concentrations of recombinant human IGF1. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of MCF-7 cells after incubation with IGF1 for 72h observed by inverted microscope was shown in Figure 2. Cell viability was assessed by CCK-8 assay after incubation with recombinant IGF1 for 72h. The result was shown in Figure 3. It was obvious that IGF1 significantly increased cell viability of MCF-7 cells.(A) MCF-7 cells cultured in DMEM, stimulated with 10ng/mL IGF1 for 72h; (B) Unstimulated MCF7 cells cultured in DMEM for 72h.Figure. Cell proliferation of MCF-7 cells after stimulated with IGF1.