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Proteine und Peptide

Human Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1), Active Protein

Produktgrößen
50 ug
£1036,00
RPU54970-50UG
100 ug
£1265,00
RPU54970-100UG
1 mg
£5260,00
RPU54970-1MG
Über dieses Produkt
SKU:
RPU54970
Zusätzliche Namen:
ALDC|Aldehyde dehydrogenase, cytosolic|ALDH1|ALDH11|PUMB1|RALDH1|Retinal dehydrogenase 1|Retinaldehyde Dehydrogenase 1
Anwendung:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Weitere Details:
Human Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1), Active Protein is a purified Prokaryotic Protein.
Formulierung:
Freeze-dried powder
Immunogen:
Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1)
Molekulargewicht:
58kDa
Physischer Zustand:
freeze-dried
Reinheit:
>95%
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles.
Hersteller:
Biomatik
Typ:
Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
Catalog Protein.JPG
ALDH1A1 (Retinal dehydrogenase 1) which belongs to the aldehyde dehydrogenase family, is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. ALDH1A1 converts retinaldehyde to retinoic acid. IL21 (interleukin 21) was identified as an interactor of ALDH1A1 through Affinity Capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human ALDH1A1 and recombinant human IL21. Briefly, ALDH1A1 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL ALDH1A1 were then transferred to IL21-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-ALDH1A1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ALDH1A1 and IL21 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure 2. SDS-PAGE