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Proteine und Peptide

Human Indoleamine-2,3-Dioxygenase (IDO), Active Protein

Produktgrößen
50 ug
£936,00
RPU54927-50UG
100 ug
£1140,00
RPU54927-100UG
1 mg
£4707,00
RPU54927-1MG
Über dieses Produkt
SKU:
RPU54927
Zusätzliche Namen:
CD107B|INDO|Indoleamine-Pyrrole 2,3 Dioxygenase
Anwendung:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Weitere Details:
Human Indoleamine-2,3-Dioxygenase (IDO), Active Protein is a purified Prokaryotic Protein.
Formulierung:
Freeze-dried powder
Immunogen:
Indoleamine-2,3-Dioxygenase (IDO)
Molekulargewicht:
43kDa
Physischer Zustand:
freeze-dried
Reinheit:
>97%
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles.
Hersteller:
Biomatik
Typ:
Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
Catalog Protein.JPG
Active Indoleamine-2,3-Dioxygenase (IDO), RPU54927
IDO (Indoleamine 2,3-dioxygenase 1) is a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. Thus, bioactivity of recombinant human IDO was measured through its ability to oxidize L-tryptophan to N-formyl-kynurenine, using Methylene Blue as indicator. Briefly, a mixture of 800uM L-tryptophan, 9000 units/mL catalase, and 40uM Methyene Blue with the addition of 80mM ascorbic acid reaction buffer was incubated with different concentrations of IDO, and the absorbance was read in 321nm every 1.5h. The result indicated that recombinant human IDO oxidize L-tryptophan.
Figure 3. SDS-PAGE