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Proteine und Peptide

Human Glutathione S Transferase Pi (GSTp), Active Protein

Produktgrößen
50 ug
£694,00
RPU54916-50UG
100 ug
£1081,00
RPU54916-100UG
1 mg
£3961,00
RPU54916-1MG
Über dieses Produkt
SKU:
RPU54916
Zusätzliche Namen:
DFN7|FAEES3|GST-P1|GST3|GSTP1
Anwendung:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Weitere Details:
Human Glutathione S Transferase Pi (GSTp), Active Protein is a purified Prokaryotic Protein.
Formulierung:
Freeze-dried powder
Immunogen:
Glutathione S Transferase Pi (GSTp)
Molekulargewicht:
25kDa
Physischer Zustand:
freeze-dried
Reinheit:
>95%
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles.
Hersteller:
Biomatik
Typ:
Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
Catalog Protein.JPG
GSTp (Glutathione S-transferase P) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 is identified as a CDK5 (Cyclin dependent kinase-5) regulatory protein, and is thought to regulates negatively CDK5 activity via p25/p35 translocation. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human GSTp and recombinant human CDK5. Briefly, GSTp were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CDK5-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-GSTp pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of GSTp and CDK5 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure 2. SDS-PAGE