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Proteine und Peptide

Human Macrophage Inflammatory Protein 1 Alpha (MIP1a), Active Protein

Produktgrößen
50 ug
£897,00
RPU54802-50UG
100 ug
£1092,00
RPU54802-100UG
1 mg
£4492,00
RPU54802-1MG
Über dieses Produkt
SKU:
RPU54802
Zusätzliche Namen:
CCL3|Chemokine C-C-Motif Ligand 3|Homologous To Mouse Mip-1a|MIP1-A|SCYA3|Small Inducible Cytokine A3|Tonsillar Lymphocyte LD78 Alpha Protein
Anwendung:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Weitere Details:
Human Macrophage Inflammatory Protein 1 Alpha (MIP1a), Active Protein is a purified Eukaryotic Protein.
Formulierung:
Freeze-dried powder
Immunogen:
Macrophage Inflammatory Protein 1 Alpha (MIP1a)
Molekulargewicht:
14kDa
Physischer Zustand:
freeze-dried
Reinheit:
>95%
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles.
Hersteller:
Biomatik
Typ:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
MIP-1a (macrophage inflammatory protein 1-alpha) also known as Chemokine (C-C motief) ligand 3 (CCL3), is a cytokine belonging to the CC chemokine family that is involved in the recruitment and activation of macrophages, monocytes and neutrophils. In this case, chemotaxis assay used 24-well microchemotaxis system was undertaken to evaluate the chemotactic effect of MIP-1a on the human monocytic cell line THP1. Briefly, THP1 cells were seeded into the upper chambers (100ul cell suspension, 106 cells/ml in RPMI 1640 with 0.5% FBS) and MIP-1a (100ng/mL, diluted in serum free RPMI 1640 ) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the Two compartments. After incubation at 37oC with 5% CO2 for 5h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (x40) and the number of migrated cells were counted at high magnification (x400) randomly (five fields for each filter). By counting migrated cells in low chamber at high magnification (x400) randomly, it was shown that a mean of 41.2 THP1 cells/field migrated towards serum free RPMI 1640 medium with 100ng/mL MIP-1a, while only 3.6 THP1 cells/field migrated towards serum free RPMI 1640 medium. And the migrated THP1 cells in low chamber at low magnification (x40) was shown in Figure 1.
Figure 3. SDS-PAGE
Figure 3. SDS-PAGE