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Proteine und Peptide

Human Monocyte Chemotactic Protein 4 (MCP4), Active Protein

Produktgrößen
50 ug
£488,00
RPU54788-50UG
100 ug
£583,00
RPU54788-100UG
1 mg
£2239,00
RPU54788-1MG
Über dieses Produkt
SKU:
RPU54788
Zusätzliche Namen:
CCL13|Chemokine C-C-Motif Ligand 13|CK-beta-10|CKb10|Monocyte chemoattractant 4|NCC1|SCYA13|SCYL1|Small Inducible Cytokine Subfamily A(Cys-Cys)Member 13
Anwendung:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Weitere Details:
Human Monocyte Chemotactic Protein 4 (MCP4), Active Protein is a purified Eukaryotic Protein.
Formulierung:
Freeze-dried powder
Immunogen:
Monocyte Chemotactic Protein 4 (MCP4)
Molekulargewicht:
13kDa
Physischer Zustand:
freeze-dried
Reinheit:
>95%
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles.
Hersteller:
Biomatik
Typ:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
Active Monocyte Chemotactic Protein 4 (MCP4), RPU54788
CCL13 (C-C motif chemokine 13) is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, which belongs to the CC chemokine subfamily. It has been reported that CCL13 can induce chemotactic migration of THP-1 cells. Therefore, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of CCL13 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100uL cell suspension, 106 cells/mL in RPMI 1640 with 0.5% FBS) and CCL13 (50ng/mL and 100ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the Two compartments. After incubation at 37oC with 5% CO2 for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (x100) and the number of migrated cells were counted at high magnification (x400) randomly (five fields for each filter). Result: CCL13 is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (x100) were shown in Figure 1. Five fields of each chamber were randomly chosen to count the migrated cells at high magnification (x400) and the statistical data was shown in Figure 2.