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Proteine und Peptide

Human Histidine Rich Glycoprotein (HRG), Active Protein

Produktgrößen
50 ug
£671,00
RPU54778-50UG
100 ug
£833,00
RPU54778-100UG
1 mg
£3806,00
RPU54778-1MG
Über dieses Produkt
SKU:
RPU54778
Zusätzliche Namen:
Histidine-Proline Rich Glycoprotein|HPRG|HRGP|Thrombophilia Due To Elevated HRG
Anwendung:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Weitere Details:
Human Histidine Rich Glycoprotein (HRG), Active Protein is a purified Eukaryotic Protein.
Formulierung:
Freeze-dried powder
Immunogen:
Histidine Rich Glycoprotein (HRG)
Molekulargewicht:
75kDa
Physischer Zustand:
freeze-dried
Reinheit:
>97%
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles.
Hersteller:
Biomatik
Typ:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
HRG (Histidine-rich glycoprotein) is a plasma glycoprotein which is distinguished by its high content of histidine and proline. HRG binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Thus, a functional binding ELISA assay was constructed to detect the association of rhHRG with heparin. Briefly, Microtiter wells were coated with OVA-conjugated-heparin. rhHRG were diluted serially in 0.01M PBS (pH 7.4). Duplicate samples of 100uL were then transferred to the coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-HRG PAb, then aspirated and washed 3 times. After incubation with HRP labeled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate Solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of rhHRG with heparin was shown in Figure 1 and this effect was in a dose dependent manner.
Figure 3. SDS-PAGE
Figure 3. SDS-PAGE