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Antikörper

Bcl2L1 Polyclonal Antibody

Produktgrößen
50 ug
£302,00
CAC11764-50UG
100 ug
£454,00
CAC11764-100UG
Über dieses Produkt
SKU:
CAC11764
Zusätzliche Namen:
Apoptosis regulator Bcl X|Apoptosis regulator Bcl-X|Apoptosis regulator BclX|B cell lymphoma 2 like|B2CL1_HUMAN|Bcl 2 like 1 protein|Bcl X|Bcl xL|BCL XL/S|Bcl xS|Bcl-2-like protein 1|Bcl2 Like 1|Bcl2 related gene|Bcl2-L-1|BCL2L|Bcl2l1|BCLX|BclXL|BclXs|DKFZp781P2092|PPP1R52|Protein phosphatase 1 regulatory subunit 52
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Klonalität:
Polyclonal
Weitere Details:
The Bcl2L1 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Host:
Rabbit
Immunogen:
BCL2L1
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Mouse
Versandbedingungen:
Blue Ice
Quelle:
Rabbit
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Biomatik
Typ:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
Western Blot; Positive WB detected in: RAW264.7 whole cell lysate; All lanes: BCL2L1 antibody at 2.7ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 27, 19, 26 kDa; Observed band size: 27 kDa
IHC image diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of MCF-7 cells with CAC11764 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).