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Antikörper

Aifm1 Polyclonal Antibody

Produktgrößen
50 ug
£302,00
CAC11738-50UG
100 ug
£454,00
CAC11738-100UG
Über dieses Produkt
SKU:
CAC11738
Zusätzliche Namen:
1|AIFM1|AIFM1_HUMAN|Apoptosis inducing factor|Apoptosis inducing factor 1|Apoptosis-inducing factor 1|axonal|axonal motor-sensory|CMTX4|COWCK|COXPD6|Harlequin|Hq|mAIF|MGC111425|MGC5706|mitochondrial|mitochondrion associated|motor-sensory with deafness and mental retardation (Cowchock syndrome)|Neuropathy|PDCD 8|PDCD8|Programmed cell death 8|Programmed cell death 8 (apoptosis inducing factor)|Programmed cell death 8 isoform 1|Programmed cell death 8 isoform 2|Programmed cell death 8 isoform 3|Programmed cell death protein 8|Programmed cell death protein 8 mitochondrial|Programmed cell death protein 8 mitochondrial precursor|Striatal apoptosis inducing factor|with deafness and mental retardation
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Klonalität:
Polyclonal
Weitere Details:
The Aifm1 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Host:
Rabbit
Immunogen:
AIFM1
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Versandbedingungen:
Blue Ice
Quelle:
Rabbit
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Biomatik
Typ:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
Western Blot; Positive WB detected in: Jurkat whole cell lysate, A549 whole cell lysate, Hela whole cell lysate; All lanes: AIFM1 antibody at 2.8ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 67, 36, 29, 27 kDa; Observed band size: 67 kDa
IHC image diluted at 1:1400 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:1400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CAC11738 at 1:466, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).