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Antikörper

Cd300Lg Polyclonal Antibody

Produktgrößen
50 ug
£302,00
CAC11333-50UG
100 ug
£454,00
CAC11333-100UG
Über dieses Produkt
SKU:
CAC11333
Zusätzliche Namen:
CD300 antigen-like family member G|CD300 molecule like family member g|CD300g|Cd300lg|CLM-9|CLM9|CLM9_HUMAN|CMRF35-like molecule 9|Nepmucin|TREM-4|TREM4|Triggering receptor expressed on myeloid cells 4
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Klonalität:
Polyclonal
Weitere Details:
The Cd300Lg Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Host:
Rabbit
Immunogen:
CD300LG
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Rat
Versandbedingungen:
Blue Ice
Quelle:
Rabbit
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Biomatik
Typ:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
Western Blot; Positive WB detected in: Rat kidney tissue; All lanes: CD300LG antibody at 6ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 37, 27, 25, 34, 30 kDa; Observed band size: 37 kDa
Immunofluorescence staining of 293 cells with CAC11333 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image diluted at 1:200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.