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Antikörper

Hist1H1E (Ab-17) Polyclonal Antibody

Produktgrößen
50 ul
£337,00
CAC11304-50UL
100 ul
£477,00
CAC11304-100UL
Über dieses Produkt
SKU:
CAC11304
Zusätzliche Namen:
H1 histone family member 4|H1.4|H14_HUMAN|H1E|H1F4|Hist1h1e|Histone 1 H1e|Histone cluster 1 H1e|Histone H1|Histone H1.4|Histone H1B|MGC116819
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Klonalität:
Polyclonal
Weitere Details:
The Hist1H1E (Ab-17) Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Host:
Rabbit
Immunogen:
HIST1H1E
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Affinity Purified
Reaktivitäten:
Rat
Versandbedingungen:
Blue Ice
Quelle:
Rabbit
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Biomatik
Typ:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
Western Blot; Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Rat brain tissue; All lanes: HIST1H1E antibody at 1.48ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 22 kDa; Observed band size: 28 kDa
IHC image diluted at 1:50 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC11304 at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).