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Antikörper

Csnk1D Polyclonal Antibody

Produktgrößen
50 ug
£302,00
CAC08341-50UG
100 ug
£454,00
CAC08341-100UG
Über dieses Produkt
SKU:
CAC08341
Zusätzliche Namen:
Casein Kinase 1 delta|Casein kinase I delta isoform|Casein kinase I isoform delta|CKI delta|CKI-delta|CKId|CKIdelta|CSNK 1D|CSNK1D|HCKID|KC1D_HUMAN|Protein kinase CK1 delta
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Klonalität:
Polyclonal
Weitere Details:
The Csnk1D Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF, IP.
Host:
Rabbit
Immunogen:
CSNK1D
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Mouse, Rat
Versandbedingungen:
Blue Ice
Quelle:
Rabbit
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Biomatik
Typ:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
Western Blot; Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate, 293T whole cell lysate, Rat brain tissue, Mouse liver tissue; All lanes: CSNK1D antibody at 5ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 48, 47 kDa; Observed band size: 48 kDa
IHC image diluted at 1:1000 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CAC08341 at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating CSNK1D in Hela whole cell lysate; , Lane 1: Rabbit control IgG (1ug) instead in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC08341 (6ug) + Hela whole cell lysate (500ug); , Lane 3: Hela whole cell lysate (10ug)