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Antikörper

Cast Polyclonal Antibody

Produktgrößen
50 ug
£302,00
CAC08287-50UG
100 ug
£454,00
CAC08287-100UG
Über dieses Produkt
SKU:
CAC08287
Zusätzliche Namen:
BS 17|Calpain inhibitor|Calpastatin|Cast|Heart type calpastatin|ICAL_HUMAN|MGC9402|Sperm BS 17 component|Sperm BS-17 component
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Klonalität:
Polyclonal
Weitere Details:
The Cast Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Host:
Rabbit
Immunogen:
CAST
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Mouse
Versandbedingungen:
Blue Ice
Quelle:
Rabbit
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Biomatik
Typ:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
Western Blot; Positive WB detected in: HL60 whole cell lysate, Mouse heart tissue; All lanes: CAST antibody at 2.6ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 77, 76, 64, 72, 82, 85, 83, 75, 81 kDa; Observed band size: 77 kDa
IHC image diluted at 1:500 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:500 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with CAC08287 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).