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Antikörper

Aqp4 Polyclonal Antibody

Produktgrößen
50 ug
£302,00
CAC07118-50UG
100 ug
£454,00
CAC07118-100UG
Über dieses Produkt
SKU:
CAC07118
Zusätzliche Namen:
AQP 4|AQP-4|AQP4|AQP4_HUMAN|Aquaporin type 4|Aquaporin-4|Aquaporin4|HMIWC 2|HMIWC2|Mercurial insensitive water channel|Mercurial-insensitive water channel|MGC22454|MIWC|WCH 4|WCH4
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Klonalität:
Polyclonal
Weitere Details:
The Aqp4 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Host:
Rabbit
Immunogen:
AQP4
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Mouse
Versandbedingungen:
Blue Ice
Quelle:
Rabbit
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Biomatik
Typ:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
Western blot; All lanes: AQP4 antibody at 2ug/ml; Lane 1: Mouse heart tissue; Lane 2: Mouse brain tissue; Lane 3: Mouse kidney tissue; Secondary; Goat polyclonal to rabbit IgG at 1/10000 dilution; Predicted band size: 35, 33 kDa; Observed band size: 35 kDa
IHC image diluted at 1:370 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:370 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of U251 cells with CAC07118 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).