THUNDER Phospho-4EBP1 (T37/T46) + Total 4EBP1 TR-FRET Cell Signaling Assay Kit
Produktgrößen
500 points (400 points for phospho; 100 points for total)
£1289,00
KIT-4EBP1PT-500-500POINTS
Über dieses Produkt
- SKU:
- KIT-4EBP1PT-500
- Anwendung:
- Cell-based/Functional Assay
- Weitere Details:
- Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-4EBP1 (T37/T46) and Total 4EBP1 in cell lysates
- Versandbedingungen:
- Dry Ice
- Lagerbedingungen:
- at -80oC
- Hersteller:
- BioAuxilium
- Typ:
- Assay Kits
- Kit complementary reagents:
- 4EBP1 Control lysate: LYS-4EBP1-100 (100 µL) LYS-4EBP1-500 (500 µL) Lysis Bufer #2 (5X; 10 mL): TRFRET-LB2 Detection Buffer (10X; 1 mL): TRFRET-DB
- Assay type:
- Semi-quantitative; homogeneous (no-wash) sandwich immunoassay
- Assay format:
- Half-area 96-well or low-volume 384-well plate
- Assay target:
- Phospho-4EBP1 (T37/T46) & Total 4EBP1
- Components:
- Eu-phospho-4EBP1 (20 µL) Acc-phospho-4EBP1 (80 µL) Eu-Total 4EBP1 (5 µL) Acc-Total 4EBP1 (20 µL) 4EBP1 control lysate (200 µL) 5X Lysis Buffer 2 (5 mL) 10X TR-FRET Detection buffer (250 µL) 100X Phosphatase Inhibitor Cocktail (250 µL)