CLOCK Recombinant Protein
Produktgrößen
10ug
£251,00
OPCD02265-10UG
50ug
£478,00
OPCD02265-50UG
200ug
£853,00
OPCD02265-200UG
Über dieses Produkt
- SKU:
- OPCD02265
- Zusätzliche Namen:
- bHLHe8;circadian locomoter output cycles kaput protein;circadian locomoter output cycles protein kaput;class E basic helix-loop-helix protein 8;clock homolog;KAT13D.
- Konjugat:
- Unconjugated
- Konzentration:
- 200 ug/mL (prior to lyoph)
- Weitere Details:
- Transcriptional activator which forms a core component of the circadian clock. The circadian clock; an internal time-keeping system; regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expression; which are translated into rhythms in metabolism and behavior. It is derived from the Latin roots 'circa' (about) and 'diem' (day) and acts as an important regulator of a wide array of physiological functions including metabolism; sleep; body temperature; blood pressure; endocrine; immune; cardiovascular; and renal function. Consists of two major components: the central clock; residing in the suprachiasmatic nucleus (SCN) of the brain; and the peripheral clocks that are present in nearly every tissue and organ system. Both the central and peripheral clocks can be reset by environmental cues; also known as Zeitgebers (German for 'timegivers'). The predominant Zeitgeber for the central clock is light; which is sensed by retina and signals directly to the SCN. The central clock entrains the peripheral clocks through neuronal and hormonal signals; body temperature and feeding-related cues; aligning all clocks with the external light/dark cycle. Circadian rhythms allow an organism to achieve temporal homeostasis with its environment at the molecular level by regulating gene expression to create a peak of protein expression once every 24 hours to control when a particular physiological process is most active with respect to the solar day. Transcription and translation of core clock components (CLOCK; NPAS2; ARNTL/BMAL1; ARNTL2/BMAL2; PER1; PER2; PER3; CRY1 and CRY2) plays a critical role in rhythm generation; whereas delays imposed by post-translational modifications (PTMs) are important for determining the period (tau) of the rhythms (tau refers to the period of a rhythm and is the length; in time; of one complete cycle). A diurnal rhythm is synchronized with the day/night cycle; while the ultradian and infradian rhythms have a period shorter and longer than 24 hours; respectively. Disruptions in the circadian rhythms contribute to the pathology of cardiovascular diseases; cancer; metabolic syndromes and aging. A transcription/translation feedback loop (TTFL) forms the core of the molecular circadian clock mechanism. Transcription factors; CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2; form the positive limb of the feedback loop; act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes); harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G; which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription; respectively. Regulates the circadian expression of ICAM1; VCAM1; CCL2; THPO and MPL and also acts as an enhancer of the transactivation potential of NF-kappaB. Plays an important role in the homeostatic regulation of sleep. The CLOCK-ARNTL/BMAL1 heterodimer regulates the circadian expression of SERPINE1/PAI1; VWF; B3; CCRN4L/NOC; NAMPT; DBP; MYOD1; PPARGC1A; PPARGC1B; SIRT1; GYS2; F7; NGFR; GNRHR; BHLHE40/DEC1; ATF4; MTA1; KLF10 and also genes implicated in glucose and lipid metabolism. Promotes rhythmic chromatin opening; regulating the DNA accessibility of other transcription factors. The CLOCK-ARNTL2/BMAL2 heterodimer activates the transcription of SERPINE1/PAI1 and BHLHE40/DEC1. The preferred binding motif for the CLOCK-ARNTL/BMAL1 heterodimer is 5'-CACGTGA-3'; which contains a flanking Ala residue in addition to the canonical 6-nucleotide E-box sequence (PubMed:23229515). CLOCK specifically binds to the half-site 5'-CAC-3'; while ARNTL binds to the half-site 5'-GTGA-3' (PubMed:23229515). The CLOCK-ARNTL/BMAL1 heterodimer also recognizes the non-canonical E-box motifs 5'-AACGTGA-3' and 5'-CATGTGA-3' (PubMed:23229515). CLOCK has an intrinsic acetyltransferase activity; which enables circadian chromatin remodeling by acetylating histones and nonhistone proteins; including its own partner ARNTL/BMAL1. Represses glucocorticoid receptor NR3C1/GR-induced transcriptional activity by reducing the association of NR3C1/GR to glucocorticoid response elements (GREs) via the acetylation of multiple lysine residues located in its hinge region (PubMed:21980503). The acetyltransferase activity of CLOCK is as important as its transcription activity in circadian control. Acetylates metabolic enzymes IMPDH2 and NDUFA9 in a circadian manner. Facilitated by BMAL1; rhythmically interacts and acetylates argininosuccinate synthase 1 (ASS1) leading to enzymatic inhibition of ASS1 as well as the circadian oscillation of arginine biosynthesis and subsequent ureagenesis (PubMed:28985504).
- Gendetails:
- clock circadian regulator
- Host:
- E. coli
- Molekulargewicht:
- 44kDa
- Proteindetails:
- Circadian locomoter output cycles protein kaput
- Reinheit:
- > 95%
- Versandbedingungen:
- Blue Ice
- Quelle:
- E.coli
- Lagerbedingungen:
- 2Â[o]C to 8Â[o]C|-80Â[o]C
- Hersteller:
- Aviva Systems Biology
- Typ:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
- Datenblatt des Herstellers:html_datasheet.php