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Antikörper

FMR1 Antibody

Produktgrößen
100 uL
£446,00
OAAJ07901-100UL
Über dieses Produkt
SKU:
OAAJ07901
Zusätzliche Namen:
POF; FMRP; POF1; FRAXA
Klonalität:
Polyclonal
Konzentration:
1 mg/mL
Weitere Details:
Multifunctional polyribosome-associated RNA-binding protein that plays a central role in neuronal development and synaptic plasticity through the regulation of alternative mRNA splicing; mRNA stability; mRNA dendritic transport and postsynaptic local protein synthesis of a subset of mRNAs. Plays a role in the alternative splicing of its own mRNA. Plays a role in mRNA nuclear export . Together with export factor NXF2; is involved in the regulation of the NXF1 mRNA stability in neurons . Stabilizes the scaffolding postsynaptic density protein DLG4/PSD-95 and the myelin basic protein (MBP) mRNAs in hippocampal neurons and glial cells; respectively; this stabilization is further increased in response to metabotropic glutamate receptor (mGluR) stimulation . Plays a role in selective delivery of a subset of dendritic mRNAs to synaptic sites in response to mGluR activation in a kinesin-dependent manner . Plays a role as a repressor of mRNA translation during the transport of dendritic mRNAs to postnyaptic dendritic spines. Component of the CYFIP1-EIF4E-FMR1 complex which blocks cap-dependent mRNA translation initiation . Represses mRNA translation by stalling ribosomal translocation during elongation . Reports are contradictory with regards to its ability to mediate translation inhibition of MBP mRNA in oligodendrocytes. Also involved in the recruitment of the RNA helicase MOV10 to a subset of mRNAs and hence regulates microRNA (miRNA)-mediated translational repression by AGO2. Facilitates the assembly of miRNAs on specific target mRNAs. Plays also a role as an activator of mRNA translation of a subset of dendritic mRNAs at synapses. In response to mGluR stimulation; FMR1-target mRNAs are rapidly derepressed; allowing for local translation at synapses . Binds to a large subset of dendritic mRNAs that encode a myriad of proteins involved in pre- and postsynaptic functions. Binds to 5'-ACU[GU]-3' and/or 5'-[AU]GGA-3' RNA consensus sequences within mRNA targets; mainly at coding sequence (CDS) and 3'-untranslated region (UTR) and less frequently at 5'-UTR. Binds to intramolecular G-quadruplex structures in the 5'- or 3'-UTRs of mRNA targets. Binds to G-quadruplex structures in the 3'-UTR of its own mRNA. Binds also to RNA ligands harboring a kissing complex (kc) structure; this binding may mediate the association of FMR1 with polyribosomes. Binds mRNAs containing U-rich target sequences. Binds to a triple stem-loop RNA structure; called Sod1 stem loop interacting with FMRP (SoSLIP); in the 5'-UTR region of superoxide dismutase SOD1 mRNA. Binds to the dendritic; small non-coding brain cytoplasmic RNA 1 (BC1); which may increase the association of the CYFIP1-EIF4E-FMR1 complex to FMR1 target mRNAs at synapses . Associates with export factor NXF1 mRNA-containing ribonucleoprotein particles (mRNPs) in a NXF2-dependent manner . Binds to a subset of miRNAs in the brain. May associate with nascent transcripts in a nuclear protein NXF1-dependent manner. In vitro; binds to RNA homopolymer; preferentially on poly(G) and to a lesser extent on poly(U); but not on poly(A) or poly(C). Moreover; plays a role in the modulation of the sodium-activated potassium channel KCNT1 gating activity. Negatively regulates the voltage-dependent calcium channel current density in soma and presynaptic terminals of dorsal root ganglion (DRG) neurons; and hence regulates synaptic vesicle exocytosis . Modulates the voltage-dependent calcium channel CACNA1B expression at the plasma membrane by targeting the channels for proteosomal degradation . Plays a role in regulation of MAP1B-dependent microtubule dynamics during neuronal development . Recently; has been shown to play a translation-independent role in the modulation of presynaptic action potential (AP) duration and neurotransmitter release via large-conductance calcium-activated potassium (BK) channels in hippocampal and cortical excitatory neurons. Finally; FMR1 may be involved in the control of DNA damage response (DDR) mechanisms through the regulation of ATR-dependent signaling pathways such as histone H2AFX/H2A.x and BRCA1 phosphorylations.
Host:
Rabbit
Immunogen:
Recombinant protein of human FMR1
Molekulargewicht:
71 kDa
Proteindetails:
Synaptic functional regulator FMR1
Aufreinigung:
Immunogen affinity chromatography
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4C. For long term storage; aliquot and store at -20C. Avoid freeze/thaw cycles.
Hersteller:
Aviva Systems Biology
Typ:
Antibodies: Polyclonal Antibody