Skip to content

Warenkorb

Der Warenkorb ist leer.

Gesamt (exkl. MwSt.) £0,00
Warenkorb überprüfen & bezahlen
Proteine und Peptide

Lba Cas12a (Cpf1) Nuclease

Produktgrößen
20 ul
£177,00
YXX26701-20UL
200 ul
£767,00
YXX26701-200UL
Über dieses Produkt
SKU:
YXX26701
Zusätzliche Namen:
CRISPR-associatedendonucleaseCas12a,CRISPR-associatedendonucleaseCpf1,LbaCas12a(Cpf1),LbaCas12a,Cpf1,LbCas12a
Weitere Details:
LbaCas12anucleaseisderivedfromLachnospiraceaebacteriumND2006andisasite-specificDNAendonucleasemediatedbyasingle-guideRNA(gRNA)withalengthof41-44nucleotides.InthepresenceofaPAM(TTTN)sequenceonthetargetdouble-strandedDNA,LbCas12aspecificallycleavesthetargetdsDNA,resultingindouble-strandbreaksandthegenerationofstickyends.Incontrast,thespecificcleavageofsingle-strandedDNAtargetsbyLbCas12adoesnotrequireaPAMsequence.\r\n LbaCas12acontainssimianvirus40(SV40)Tantigennuclearlocalizationsequences(NLS)atboththeN-terminusandC-terminus.WhenCas12aisexpressedwiththeseNLSsequences,theCas12a-RNPcomplexcanrapidlylocalizetothenucleusuponcellularentry.\r\n Additionally,bothdouble-strandedandsingle-strandedDNAtargetscanactivatethetrans-cleavageactivityofLbCas12a.Specifically,uponformationofaternarycomplexamongtheLbCas12aenzyme,crRNA,andtargetDNA,theenzymebecomesactivatedandexhibitsnon-specifictrans-cleavageactivity,degradinganysingle-strandedDNA(ssDNA)presentinthesystem.Consequently,LbCas12acannotonlybeusedforspecificcleavageofdsDNAinvitrobutalsoenablesrapiddetectionoftargetnucleicacids.
Physischer Zustand:
Liquid
Reinheit:
>90% as determined by sds-page.
Versandbedingungen:
Dry Ice
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles.
Hersteller:
AntibodySystem
Typ:
Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes