Polyclonal Antibody to RNF8 (Ab-137)
Produktgrößen
50 µl
£511,00
35-1867-50UL
100 µl
£581,00
35-1867-100UL
Über dieses Produkt
- SKU:
- 35-1867
- Zusätzliche Namen:
- KIAA0646
- Klonalität:
- Polyclonal
- Weitere Details:
- E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites; and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites. Following DNA DSBs; it is recruited to the sites of damage by ATM-phosphorylated MDC1 and catalyzes the 'Lys-63'-linked ubiquitination of histones H2A and H2AX; thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). Also controls the recruitment of UIMC1-BRCC3 (RAP80-BRCC36) and PAXIP1/PTIP to DNA damage sites. Also recruited at DNA interstrand cross-links (ICLs) sites and catalyzes 'Lys-63'-linked ubiquitination of histones H2A and H2AX; leading to recruitment of FAAP20/C1orf86 and Fanconi anemia (FA) complex; followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis; a mechanism to avoid collision between transcription and repair intermediates. Promotes the formation of 'Lys-63'-linked polyubiquitin chains via interactions with the specific ubiquitin-conjugating UBE2N/UBC13 and ubiquitinates non-histone substrates such as PCNA. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation. Also catalyzes the formation of 'Lys-48'-linked polyubiquitin chains via interaction with the ubiquitin-conjugating UBE2L6/UBCH8; leading to degradation of substrate proteins such as CHEK2; JMJD2A/KDM4A and KU80/XRCC5: it is still unclear how the preference toward 'Lys-48'- versus 'Lys-63'-linked ubiquitination is regulated but it could be due to RNF8 ability to interact with specific E2 specific ligases. For instance; interaction with phosphorylated HERC2 promotes the association between RNF8 and UBE2N/UBC13 and favors the specific formation of 'Lys-63'-linked ubiquitin chains. Promotes non-homologous end joining (NHEJ) by promoting the 'Lys-48'-linked ubiquitination and degradation the of KU80/XRCC5. Following DNA damage; mediates the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF168; leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. In addition to its function in damage signaling; also plays a role in higher-order chromatin structure by mediating extensive chromatin decondensation. Involved in the activation of ATM by promoting histone H2B ubiquitination; which indirectly triggers histone H4 'Lys-16' acetylation (H4K16ac); establishing a chromatin environment that promotes efficient activation of ATM kinase. Required in the testis; where it plays a role in the replacement of histones during spermatogenesis. At uncapped telomeres; promotes the joining of deprotected chromosome ends by inducing H2A ubiquitination and TP53BP1 recruitment; suggesting that it may enhance cancer d
- Format:
- Purified
- Host:
- Rabbit
- Immunogen:
- Peptide sequence around aa.135~139 (C-L-S-P-K) derived from Human RNF8.
- Isotyp:
- Rabbit IgG
- Reaktivitäten:
- Human
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- Store the antibody at 4[o]C; stable for 6 months. For long-term storage; store at -20[o]C. Avoid repeated freeze and thaw cycles.
- Hersteller:
- Abeomics
- Typ:
- Antibodies:Polyclonal Antibody
- Datenblatt des Herstellers:1.jpg