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Antikörper

Polyclonal Antibody to Brg1

Produktgrößen
50 µl
£511,00
35-1830-50UL
100 µl
£581,00
35-1830-100UL
Über dieses Produkt
SKU:
35-1830
Zusätzliche Namen:
BRG1|| SNF2|| SWI2|| RTPS2|| BAF190
Klonalität:
Polyclonal
Weitere Details:
Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex; a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons; transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx; RB1 is dephosphorylated by calcineurin; which leads to release of the repressor complex. At the same time; there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism; which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter; and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons; npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A; are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals; while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex; a chromatin-remodeling complex recruited by vitamin D receptor (VDR); which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1.
Format:
Purified
Host:
Rabbit
Immunogen:
Peptide sequence around aa.701~705(V-D-A-R-H) derived from Human Brg1.
Isotyp:
Rabbit IgG
Reaktivitäten:
Human, Mouse, Rat
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store the antibody at 4[o]C; stable for 6 months. For long-term storage; store at -20[o]C. Avoid repeated freeze and thaw cycles.
Hersteller:
Abeomics
Typ:
Antibodies:Polyclonal Antibody
  • Datenblatt des Herstellers:1.jpg