Recombinant Human Tumor Necrosis Factor a/TNFa (C-6His)
Produktgrößen
10 µg
£448,00
32-8282-10UG
50 µg
£508,00
32-8282-50UG
Über dieses Produkt
- SKU:
- 32-8282
- Weitere Details:
- Source: E. coli. MW :18.5kD. Recombinant Human Tumor Necrosis Factor alpha is produced by our E.coli expression system and the target gene encoding Val77-Leu233 is expressed with a 6His tag at the C-terminus. Tumor Necrosis Factor-a (TNF-a) is secreted by macrophages; monocytes; neutrophils; T-cells; and NK-cells following stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-a while cells that express CD8 secrete little or no TNF-a. Synthesis of TNF-a can be induced by many different stimuli including interferons; IL2; and GM-CSF. The clinical use of the potent anti-tumor activity of TNF-a has been limited by the proinflammatory side effects such as fever; dose-limiting hypotension; hepatotoxicity; intravascular thrombosis; and hemorrhage. Designing clinically applicable TNF-a mutants with low systemic toxicity has been of intense pharmacological interest. Human TNF-a that binds to murine TNF-R55 but not murine TNF-R7; exhibits retained anti-tumor activity and reduced systemic toxicity in mice compared with murine TNF-a; which binds to both murine TNF receptors. Based on these results; many TNF-a mutants that selectively bind to TNF-R55 have been designed. These mutants displayed cytotoxic activities on tumor cell lines in vitro and have exhibited lower systemic toxicity in vivo. Recombinant Human TNF-a High Active Mutant differs from the wild-type by amino acid subsitution of amino acids 1-7 with Arg8; Lys9; Arg10 and Phe157. This mutant form has been shown to have increased activity with less inflammatory side effects in vivo.
- Versandbedingungen:
- Ambient
- Lagerbedingungen:
- Lyophilized protein should be stored at -20[o]C; though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7[o]C for 2-7 days. Aliquots of reconstituted samples are stable at -20[o]C for 3 months.
- Hersteller:
- Abeomics
- Typ:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins
- Datenblatt des Herstellers:download_pdf.php