Recombinant Protein A/G
Produktgrößen
10 mg
£528,00
32-4506-10MG
Über dieses Produkt
- SKU:
- 32-4506
- Weitere Details:
- Source : Escherichia coli. The recombinant Protein A/G consists of 7 IgG-binding domains EDABC-C2C3; which corresponds to the Protein A and G domains that are included in the recombinant sequence. The Protein A portion is from Staphylococcus aureus segments E; D; A; B and C. The Protein G portion is from Streptococcus segments C2 and C3. The fusion protein has a predicted molecular mass of 50.5 kDa. But it migrates with an apparent molecular mass of approximately 45kDa in SDS-PAGE. Recombinant Protein A/G fusion protein joins IgG binding domains of both Protein A and Protein G. Protein A/G includes four Fc binding domains from Protein A and two from Protein G; yielding a final mass of 50.4 kDa. The binding dependency to pH of Protein A/G lower than Protein A; but has the additive properties of Protein A and G together. Protein A/G binds to all subclasses of human IgG; making it helpful for purifying polyclonal or monoclonal IgG antibodies whose subclasses have not been identifieed. Protein A/G binds to IgA; IgE; IgM and IgD. Protein A/G binds to all subclasses of mouse IgG excluding mouse IgA; IgM or serum albumin. This permits Protein A/G to be used in purification and detection of mouse monoclonal IgG antibodies; with no interference from IgA; IgM and serum albumin. Mouse monoclonal antibodies normally have a stronger affinity to the chimeric Protein A/G than to either Protein A or Protein G. Protein A/G also has been used for purification of macaque IgG.
- Aufreinigung:
- >95% as determined by SDS-PAGE and RP-HPLC.
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- After reconstitution; aliquot and store at -20[o]C. Avoid repeated freeze/thaw cycles.
- Hersteller:
- Abeomics
- Typ:
- Proteins, Peptides, Small Molecules & Other Biomolecules
- Datenblatt des Herstellers:1.jpg