Recombinant E.Coli Transcription Termination/Antitermination L Factor
Produktgrößen
50 µg
£528,00
32-4359-50UG
Über dieses Produkt
- SKU:
- 32-4359
- Zusätzliche Namen:
- Transcription elongation protein nusA||N utilization substance protein A||L factor||nusA||ECK3158||JW3158||b3169||Transcription Termination/Antitermination L Factor.
- Weitere Details:
- Source : Escherichia Coli. NusA Recombinant produced in E.Coli is a single; non-glycosylated polypeptide chain containing 495 amino acids (1-495a.a.) and having a molecular mass of 54 kDa. NusA is an important player in both prevention and enhancement of transcriptional termination. NusA is important both in Rho-dependent and intrinsic termination; as well as in lambda and other phage antitermination systems. The NusA gene was first identified by isolation of the nusAl mutation; which limits bacteriophage-l growth by preventing the antitermination activity of the l N protein. NusA plays a role in transcriptional antitermination in the cell. It has been shown to specifically aid in read-through of the RNA polymerase genes rpoB and rpoC; as well as in successful synthesis of the ribosomal RNA genes. Additionally to its anti-termination role; NusA is needed for both Rho-dependent and intrinsic transcriptional termination. NusA is obligatory for Rho-dependent termination in lambda phage and in the cell. NusA plays a role in intrinsic termination and the inhibition of RNA elongation. However NusA interacts with all three subunits of RNA polymerase; its termination activity primarily depends on its interaction with the carboxy-terminus of RpoA. NusA induces conformational change in RNA polymerase & prevents RNA interaction with RpoA. This binding sequentially activates NusA; allowing it to bind RNA and promote formation of hairpins at intrinsic termination sites. NusA binds Rho; and participates with sigma70 for binding to the core RNA polymerase complex. NusA does not compete with NusG for binding to either Rho or the polymerase; despite modulating the same process as NusG in both cases.
- Aufreinigung:
- Greater than 95.0% as determined by SDS-PAGE.
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- Store at 4[o]C if entire vial will be used within 2-4 weeks. Store; frozen at -20[o]C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
- Hersteller:
- Abeomics
- Typ:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins
- Datenblatt des Herstellers:1.jpg