PPM1A Recombinant Protein
Produktgrößen
50 µg
£528,00
32-3097-50UG
Über dieses Produkt
- SKU:
- 32-3097
- Zusätzliche Namen:
- Protein phosphatase 1A||EC 3.1.3.16||Protein phosphatase 2C isoform alpha||PP2C-alpha||IA||PPM1A||PP2CA||MGC9201.
- Weitere Details:
- Source : Escherichia Coli. Protein Phosphatase 1A Alpha Isoform Human Recombinant produced in E.coli is a single; non-glycosylated polypeptide chain containing 418 amino acids and having a molecular mass of 46.6KDa (containing a 36 aa His tag; T7 gene 10 leader; XpressTM Epitope). The protein coding region of PPM1A (amino acids 1-382) was cloned into an E. coli expression vector (BamHI/Hind3 site). PP2Ca was overexpressed in E. coli as a soluble His-tag fusion protein; and it was purified by conventional column chromatographic techniques. Protein Phosphatase 2C alpha is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates; and negatively regulates the activities of; MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases; and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53; which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding two distinct isoforms have been described.Protein phosphatase 2C(PP2C) is a Mn2+- or Mg2+-dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response in eukaryotes.
- Aufreinigung:
- Greater than 95.0% as determined by SDS-PAGE.
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- Store at 4[o]C if entire vial will be used within 2-4 weeks. Store; frozen at -20[o]C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
- Hersteller:
- Abeomics
- Typ:
- Proteins, Peptides, Small Molecules & Other Biomolecules
- Datenblatt des Herstellers:1.jpg