Pfu DNA Polymerase Recombinant Protein
Produktgrößen
500U
£638,00
32-2658-500U
Über dieses Produkt
- SKU:
- 32-2658
- Zusätzliche Namen:
- DNA polymerase||EC 2.7.7.7||Pfu polymerase||Pfu-DNA Polymerase.
- Weitere Details:
- Source : Escherichia Coli. Pfu DNA Polymerase is a thermo-stable enzyme having a Mw of about 90kDa. Pfu DNA Polymerase is derived from E. coli that and cloned from Pyrococcus furiosus strain Vc1 DSM3638. Pfu DNA Polymerase replicates DNA at 75°C;; catalyzing the polymerization of nucleotides into duplex DNA in the 5' to 3' direction in the existence of magnesium. Pfu DNA Polymerase possesses 3' to 5' ´ exonuclease (proofreading) activity. Base misinsertions that take place during polymerization are swiftly removed by the proofreading activity of the polymerase. Therefore; Pfu DNA Polymerase is suggested for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated PCR fragments are blunt-ended. Pfu DNA polymeraseenzyme is found in the hyperthermophilic archaeonPyrococcus furiosus; where it functions in vivoto replicate the organism's DNA. In vitro; Pfu is used to swiftly amplify DNAin the Polymerase Chain Reaction; where the enzyme serves the central function of copying a new strand of DNA during each extension step. Pfu DNA polymerase has superior thermostability and 'proofreading' properties compared to other thermostable polymerases. Unlike Taq DNA polymerase; Pfu DNA polymerase possesses 3' to 5' exonuclease proof reading activity; meaning that it works its way along the DNA from the 5' endto the 3' endand corrects nucleotidemisin corporation errors. Pfu DNA polymerase-generated PCRfragments will have fewer errors than Taq-generated PCR inserts. As a result; Pfu is more commonly used for molecular cloning of PCR fragments than the historically popular Taq. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis; but can also be used in conjunction with Taq polymerase to obtain the fidelity of Pfu with the speed of Taq polymerase activity.
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- Pfu DNA Polymerase although stable at 10[o]C for 5 days; should be stored below -18[o]C. Please prevent freeze-thaw cycles.
- Hersteller:
- Abeomics
- Typ:
- Proteins, Peptides, Small Molecules & Other Biomolecules
- Datenblatt des Herstellers:1.jpg