M2 Recombinant Protein
Produktgrößen
20 µg
£528,00
32-2518-20UG
Über dieses Produkt
- SKU:
- 32-2518
- Weitere Details:
- Source : Sf9 insect cells. Recombinant antigen for solid (ELISA) and fluid phase diagnostic assays. Mixture of E2/dihydrolipamide acyltransferase-subunits from 3 mitochondrial protein complexes: pyruvate dehydrogenase complex (PDC-E2) having a molecular mass of 60;630 Dalton (pI 5.8); 2-oxo-glutarate dehydrogenase complex (OGDC-E2) having a molecular mass of 42;301 Dalton (pI 6.3); branched chain 2-oxo-acid dehydrogenase complex (BCOADCE2) having a molecular mass of 47;321 Dalton (pI 6.5). Mixture contains equal mass of each protein component. cDNAs coding for the mature forms of the human PDC-E2; OGDC-E2 and BCOADC-E2 proteins individually fused to a hexa-histidine purification tag. M2 autoantigen is a key mark of antimitochondrial autoantibodies (AMA); a typical serological feature in patients suffering from primary biliary cirrhosis (PBC) which is a serious autoimmune liver disease accompanied by damage to intrahepatic bile ducts. Molecular definition of the M2 antigen has displayed it as no less than 3 separate target proteins. The M2 role is like the so-called E2 subunits (or dihydrolipoamide transferases) of different mitochondrial dehydrogenase complexes: *pyruvate dehydrogenase complex. *branched chain 2-oxo-acid dehydrogenase complex. *2-oxoglutarate dehydrogenase complex. Biochemically; these complexes catalyze the oxidative decarboxylation of various alpha-keto-acid substrates and systematically engage with a prosthetic lipoamide group; they are situated in the mitochondrial matrix in association with the inner membrane. The most well-known reactivity of AMA positive PBC sera is against PDC-E2. Some patients have AMA which reacts with PDC-E2 alone (95%); but most patients also show reactivity against OGDC-E2 (39-88%) and/or BCOADC-E2 (53-55%). Actually; patients can be found with reactivity only against OGDC-E2 and/or BCOADC-E2 and no PDC-E2 autoantibodies. These patients will be overlooked in assays based on natural source-derived; predominantly PDC-E2-containing M2 antigen preparations.
- Aufreinigung:
- Greater than 75% as determined by SDS-PAGE.
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- Store at 4[o]C if entire vial will be used within 2-4 weeks. Store; frozen at -20[o]C for longer periods of time. Avoid multiple freeze-thaw cycles.
- Hersteller:
- Abeomics
- Typ:
- Proteins, Peptides, Small Molecules & Other Biomolecules
- Datenblatt des Herstellers:1.jpg