GST Recombinant Protein
Produktgrößen
50 µg
£528,00
32-2401-50UG
Über dieses Produkt
- SKU:
- 32-2401
- Zusätzliche Namen:
- Glutathione S-Transferase||GST||Glutathione S-transferase class-mu 28 kDa isozyme||GST 28||EC 2.5.1.18||Sj28GST||Sj28 antigen.
- Weitere Details:
- Source : Escherichia Coli. Recombinant Glutathione S-Transferase full length protein (1-224a.a.) expressed in E.coli; having a molecular mass of 26kDa. GST was isolated from an E. coli strain that carries the coding sequence for Schistosoma japonicum GST under the control of a T7 promoter. The GST is purified by proprietary chromatographic techniques. Antioxidant enzyme Glutathione S- Transferase (GST) is thought to do the primary cellular defense mechanism against reactive oxygen species. GST reduces lipid hydroperoxides through its Se-independent glutathione peroxidase activity. The enzyme also detoxifies lipid peroxidation end products such as 4-hydroxynonenal (4-HNE).The soluble GST is a 26 kDa protein which occurs as a dimer in all aerobic organisms. Each monomer has two domains; one that binds GSH and is an /-structure similar to thioredoxin and the other; all helical; that binds the hydrophobic substrate. The GST -fusion protein expression system is a widely used recombinant protein expression system that allows a peptide or a regulatory protein domain to be expressed as a fusion to the C-terminus of Schistosoma japonicum GST. Fusion proteins also possess GST -enzymatic activity and can undergo dimerization similar to in vivo. The fusion protein can be purified via GST -affinity column chromatography. In most cases; the desired peptides or domains are removed from GST by applying a specific protease that recognizes and cleaves the linker between the protein domain and GST. The technique has been widely used to generate different kinds of proteins for crystallization; molecular immunology studies; the production of vaccines and studies involving protein-protein and protein-DNA interactions.
- Aufreinigung:
- Greater than 95% as determined by SDS-PAGE.
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- Store at 4[o]C if entire vial will be used within 2-4 weeks. Store; frozen at -20[o]C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
- Hersteller:
- Abeomics
- Typ:
- Proteins, Peptides, Small Molecules & Other Biomolecules
- Datenblatt des Herstellers:1.jpg