Proguanylin Recombinant Protein
Produktgrößen
10 µg
£528,00
32-2068-10UG
Über dieses Produkt
- SKU:
- 32-2068
- Zusätzliche Namen:
- Guanylin Precursor||Guanylate cyclase activator 2A||Guanylate cyclase-activating protein 1||Gap-IGUCA2||STARA||GUANYLIN.
- Weitere Details:
- Source : Escherichia Coli. Proguanylin Human Recombinant produced in E. coli; is an 11 kDa protein containing 96 amino acid residues of the human proguanylin and 2 additional amino acid residues .The Proguanylin is purified by proprietary chromatographic techniques.GPVTVQDGNF SFSLESVKKL KDLQEPQEPR VGKLRNFAPI PGEPVVPILC SNPNFPEELK PLCKEPNAQE ILQRLEEIAE DPGTCEICAY AACTGC. Heat-stable enterotoxins (STa) are small; cysteine-rich peptides secreted by Escherichia coli that are able to induce diarrhea through the stimulation of an intestine-specific receptor-guanylyl cyclase known as STaR. Binding of STa to STaR induces a dramatic increase in the cGMP content of the cell; the increase; in turn; inhibits salt absorption and stimulates chloride secretion. This imbalance of ions is accompanied by a massive accumulation of water in the gut that gives rise to the diarrhea and dehydration characteristic of enterotoxin activity. The identification of a receptor for STa on intestinal brush border membranes suggested the existence of an endogenous activator; described guanylin; a 15-amino acid peptide purified from rat small intestine; as a potential ligand for the STaR. This peptide shares sequence similarity with STa; see also uroguanylin. The molecular cloning of the human and mouse cDNAs encoding guanylin was reported. The sequences demonstrated that guanylin is present at the C-terminal end of a larger precursor protein. Expression in mammalian cells indicated that the 94- amino acid proguanylin is inactive. The biologically active guanylin can be released by either chemical or enzymatic treatment of proguanylin. By Northern blot analysis and in situ hybridization; showed that expression of guanylin mRNA is restricted to cells of the intestinal epithelium; specifically the Paneth cells at the base of the small intestinal crypts. These results demonstrate that guanylin is an endogenous activator of STaR isolated a cDNA encoding an apparent precursor of guanylin from a human intestinal cDNA library. The mRNA was expressed at high levels in human ileum and colon. In the mouse; interspecific backcross analysis used to map the Guca2 gene to the distal half of mouse chromosome 4 in a region of homology with human chromosome 1p. By fluorescence in situ hybridization mapped the GUCA2 gene to human 1p35-p34 Guanylin is thought to modulate intestinal water/electrolyte transport in a paracrine mode reported the nucleotide sequence of the gene; the characteristics of its circulating molecular form; and its localization in enterochromaffin cells of the gut. The gene; approximately 2.6 kb in size; consists of 3 exons interrupted by 2 introns. The hormonal form of guanylin is a 94-amino acid peptide with a molecular mass of 10.3 kDa. Guanylin is synthesized by gut enterochromaffin cells as a prohormone of 115 amino acids and is processed to the molecular form of 94 amino acids circulating in the blood.
- Aufreinigung:
- Greater than 95.0% as determined by SDS-PAGE.
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- Store lyophilized protein at -20[o]C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4[o]C for a limited period of time; it does not show any change after two weeks at 4[o]C.
- Hersteller:
- Abeomics
- Typ:
- Proteins, Peptides, Small Molecules & Other Biomolecules
- Datenblatt des Herstellers:1.jpg