GPHA2 Recombinant Protein
Produktgrößen
10 µg
£528,00
32-2038-10UG
Über dieses Produkt
- SKU:
- 32-2038
- Zusätzliche Namen:
- GPA2||GPHA2||ZSIG51||Glycoprotein hormone alpha-2||MGC126572.
- Weitere Details:
- Source : Escherichia Coli. GPHA2 Human Recombinant produced in E.Coli is a single; non-glycosylated; polypeptide chain containing 120 amino acids and having a total molecular mass of 13.28 kDa. The Thyrostimulin contains His tag which consists of 14 additional amino acids.The amino acid sequence of the recombinant human Thyrostimulin beta subunit is 100% homologous to the amino acid sequence of the human Thyrostimulin beta subunit without signal sequence. (N-terminal 24AA).Thyrostimulin is purified by proprietary chromatographic techniques. Human thyrostimulin ranks among the glycoprotein hormone family. These hormones consist of two subunits; the common alpha- and specific beta-subunits; which associate noncovalently to form a heterodimer. The alpha-subunit combines with four distinct beta-subunits giving rise to four biologically active hormones in human: FSH; LH; TSH; and CG. FSH; LH; and TSH; mainly expressed in the anterior pituitary; are essential for coordinated endocrine regulation in the hypothalamus- pituitary axis and show to activate specific G protein-coupled receptors in the thyroid (TSH receptor) and gonads (LH and FSH receptors); respectively.The heterodimeric glycoprotein hormones have only been identified in vertebrates and are highly conserved in organisms from primitive rayfin fish (Chondrostei) to human in both primary sequences and functional characteristics.Corticotroph-derived glycoprotein hormone (CGH); also referred to as thyrostimulin; is a noncovalent heterodimer of glycoprotein hormone alpha 2 (GPHA2) and glycoprotein hormone beta 5 (GPHB5).Recombinant A2/B5 heterodimeric glycoproteins activates human TSH receptors; but not LH and FSH receptors; and shows high affinity to TSH receptors in a radioligand receptor assay. The heterodimer also stimulates cAMP production and thymidine incorporation by cultured thyroid cells and increases serum thyroxine levels in TSH-suppressed rats in vivo. This new heterodimeric glycoprotein hormone was named as thyrostimulin based on its thyroid-stimulating activity. The expression of thyrostimulin in the anterior pituitary known to express TSH receptors suggested a paracrine mechanism.
- Aufreinigung:
- Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- Lyophilized Thyrostimulin although stable at room temperature for 3 weeks; should be stored desiccated below -18[o]C. Upon reconstitution GPHA2 should be stored at 4[o]C between 2-7 days and for future use below -18[o]C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
- Hersteller:
- Abeomics
- Typ:
- Proteins, Peptides, Small Molecules & Other Biomolecules
- Datenblatt des Herstellers:1.jpg