AGRP Recombinant Protein
Produktgrößen
10 µg
£528,00
32-2006-10UG
Über dieses Produkt
- SKU:
- 32-2006
- Zusätzliche Namen:
- ART||AGRT||ASIP2||MGC118963||AGRP.
- Weitere Details:
- Source : Escherichia Coli. The Human Agouti-related protein is created as a recombinant protein with N-terminal fusion of His Tag.The Human Agouti-related protein His-Tagged Fusion Protein; produced in E. coli; is 14.4 kDa (calculated) protein containing 112 amino acid residues of the human AGRP and 16 additional amino acid residues - His Tag; thrombin cleavage site (highlighted).The AGRP is purified by proprietary chromatographic techniques.MKHHHHHHHM LVPRGSAQMG LAPMEGIRRP DQALLPELPG LGLRAPLKKT TAEQAEEDLL QEAQALAEVL DLQDREPRSS RRCVRLHESC LGQQVPCCDP CATCYCRFFN AFCYCRKLGT AMNPCSRT. Agouti-related protein is an endogenous antagonist of hypothalamic alpha-melanocortin receptors MC3R and MC4R with potent orexigenic activity. Although a complete deletion of the AGRP gene does not produce any significant metabolic phenotypes; reduction in AGRP expression by RNA interference is associated with increased metabolic rate along with reduced weight gain. In hypothalamus; it is produced by neurons in the medial portion of arcuate nucleus; which produce also the potent orexigenic peptide Neuropeptide Y (NP-Y). Another site of central AGRP production is the hypothalamic nucleus. AGRP encompasses 132 amino acid residues and its alpha-melanocortin inhibiting activity results in a 34 amino acid cystine knot domain within the C-terminal (87-132) portion of the protein. Both AGRP and NP-Y expression was shown to be suppressed by leptin. Central administration of AGRP induces hyperphagia and increased gain in body weight in rodents; but may also exert metabolic effects even when hyperphagia is prevented. In the absence of hyperphagia; intracerebralventricular administration of AGRP caused significant increases in plasma leptin and insulin concentrations (twofold and 1.5-fold; respectively) and fat pad mass.In the periphery; AGRP mRNA was found in adrenal glands; lung; testis; ovary; skeletal muscle and adipose tissue in humans or rodents. In the adrenals; it was shown that AGRP antagonizes glucosteroid production mediated by MC4R. AGRP could then modulate locally the functions of some peripheral tissues such as adrenals.In human and rat serum; detectable levels of AGRP-like activity were reported in the lower picogram range. The serum AGRP levels were elevated in obese humans compared to lean controls and increased with fasting in rats.
- Aufreinigung:
- Purity of of Agouti-related protein recombinant human is >95 % as determined by SDS-PAGE.
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- Store lyophilized AGRP protein at -20[o]C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4[o]C for a limited period of time; it does not show any change after two weeks at 4[o]C.
- Hersteller:
- Abeomics
- Typ:
- Proteins, Peptides, Small Molecules & Other Biomolecules
- Datenblatt des Herstellers:1.jpg