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Antikörper

Biotin Conjugated Anti-Human IL-2 MAb (Clone: 35C3)

Produktgrößen
0.1MG
£678,00
30-2836Biotin-0.1MG
Über dieses Produkt
SKU:
30-2836Biotin
Zusätzliche Namen:
Interleukin 2
translate.label.attr.clone:
35C3
Klonalität:
Monoclonal
Konjugat:
Biotin
Weitere Details:
IL-2 (interleukin 2) is a cytokine that is produced primarily by stimulated Th cells and its crucial role is induction of T cell proliferation. However; IL-2 also stimulates growth and differentiation of B cells; NK cells; monocytes and other cell types; such as LAK cells or oligodendrocytes and is one of the key molecules of the immune system. IL-2 signaling pathways lead to induction of Bcl-2 protein. Specificity:The mouse monoclonal antibody 35C3 recognizes human interleukin 2 (IL-2; secreted or intracellular).
Host:
Mouse
Immunogen:
Recombinant human IL-2
Isotyp:
Mouse IgG2b kappa
Aufreinigung:
Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at 2-8[o]C. Do not freeze.
Hersteller:
Abeomics
Typ:
Antibodies:Monoclonal Antibody
  • Datenblatt des Herstellers:1.jpg
Figure 2: Flow cytometry multicolor surface staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human lymphocytes using anti-human CD3 (UCHT1) Pacific Blue antibody (4 μl reagent / 100 μl of peripheral whole blood) and intracellular staining of human lymphocytes using anti-human IL-2 (35C3) Biotin antibody (concentration in sample 0.5 μg/ml; Streptavidin PE).
Figure 3: Flow cytometry multicolor surface staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human lymphocytes using anti-human CD4 (MEM-241) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and intracellular staining of human lymphocytes using anti-human IL-2 (35C3) Biotin antibody (concentration in sample 0.5 μg/ml; Streptavidin PE).
Figure 4: Separation of human CD3 positive IL-2 positive lymphocytes (red-filled) from CD3 negative IL-2 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of PMA + Ionomycin stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-2 (35C3) Biotin antibody (concentration in sample 0.5 μg/ml; Streptavidin PE).
Figure 4: Separation of human CD3 positive IL-2 positive lymphocytes (red-filled) from CD3 negative IL-2 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of PMA + Ionomycin stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-2 (35C3) Biotin antibody (concentration in sample 0.5 μg/ml; Streptavidin PE).
Figure 5: Flow cytometry intracellular staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-2 (35C3) Biotin antibody (concentration in sample 0.5 μg/ml; Streptavidin PE).