Polyclonal antibody to AIF
Produktgrößen
50 µl
£396,00
20-1113-50UL
Über dieses Produkt
- SKU:
- 20-1113
- Zusätzliche Namen:
- AIFM1||AIF||PDCD8
- Klonalität:
- Polyclonal
- Weitere Details:
- AIF (apoptosis-inducing factor) was initially discovered as a protein involved in caspase-independent cell death. It is now known that AIF has both vital and lethal functions (reviewed in Modjtahedi et al; 2006). In healthy cells; AIF is a flavoprotein present in the mitochondria where it has vital roles in cellular redox metabolism and mitochondrial bioenergetics. In many models of apoptosis; AIF is released from the mitochondria during mitochondrial outer membrane permeabilization along with other mitochondrial proteins. Upon release; AIF translocates first to the cytosol and then to the nucleus where it induces chromatin condensation and DNA degradation. Although several lines of evidence suggest that AIF is a main mediator of capsase-independent cell death; the mechanisms regulating AIF pro-apoptotic function remain to be fully elucidatied and may depend on the cell type and type of apoptotic stimuli. Human AIF is transcribed from a nuclear gene located on the X chromosome and translated in the cytoplasm to a precursor protein of 613 amino acids (aa) which corresponds to ~67 kDa. The precursor protein is imported into the mitochonria by mitochondrial localization sequences located within the N-terminal prodomain of AIF. Once inside the mitochondria; the prodomain is cleaved giving rise to a mature AIF form of ~57 kDa. AIF isoforms generated from a single AIF gene have been identified; including AIF short isoform 2 (324 aa protein; GenBank no. AAY84739.1) and AIF short isoform 3 (237 aa protein; GenBank noAAY84741.1 (reviewed in Delttre et al; 2006). The generation of multiple isoforms from a common gene is an evolutionary mechanism that increases protein diversiity in eukaryotes. Regulating gene expression through the production of multiple isoforms from a single gene is thought to play a major role in the control of apoptosis and other forms of programmed cell death. This antibody recognizes AIF and AIF isoforms containing the peptide immunogen sequence; RARDPGARVLIVSEDPELP.
- Format:
- Sera
- Host:
- Rabbit
- Immunogen:
- A synthetic peptide of human AIF (amino acids 151-169 RARDPGARVLIVSEDPELP) was used as immunogen for this antibody
- Isotyp:
- Rabbit IgG
- Reaktivitäten:
- Human, Mouse, Rat
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- Store the antibody at 4[o]C; stable for 6 months. For long-term storage; store at -20[o]C. Avoid repeated freeze and thaw cycles.
- Hersteller:
- Abeomics
- Typ:
- Antibodies:Polyclonal Antibody
- Datenblatt des Herstellers:1.jpg
