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Antikörper

Polyclonal antibody to Cre

Produktgrößen
50 µl
£396,00
20-1112-50UL
Über dieses Produkt
SKU:
20-1112
Zusätzliche Namen:
Recombinase cre
Klonalität:
Polyclonal
Weitere Details:
This antibody recognizes a 343 amino acid recombinase protein. The Cre (cyclization recombination) enzyme; a member of a large family of recombinases; recognizes loxP which is a sequence motif of 34 bp from the PI bateriophage. If a DNA seqment is flanked by two loxP sites in the same orientation; Cre excises that seqment leaving a single loxP site in the remaining DNA molecule. The excised DNA segment is subsequently degraded. Cre and lox comprise the Cre/lox system which was first developed in the late 1980's to artificially manipulate gene expression (reviewed in Branda and Dymecki; 2004; Ghosh and Duyne; 2002; and Gilbertson; 2003). As an example; mice with the Cre protein expressing in a specific cell type are bred with mice that contain a target gene surrounded by loxP sites. When the mice are bred; the cells carrying Cre will cause those cells to lose the target gene. If the Cre gene is; for example; bound to a promoter that only allows Cre production in neuronal cells; the target gene will be deleted only in those cells. Since 1989 the Cre/lox system has been used extensively and there are numerous animal; plant and bacterial stocks that already contain the Cre gene driven by ubiquitous; tissue-specific or inducible promoters and provide a quick method for breeding experiments. The Cre/lox system has the advantage of working in almost any type of cell. This has led to its application in many experiments; for example in selectively labeling neuronal cells in the brain thereby differentiating them from other types of surrounding cells.
Format:
Sera
Host:
Rabbit
Immunogen:
A synthethic peptide of the bacteriophage protein Cre (amino acids 57-73 KLNNRKWFPAEPEDVRD) was used as immunogen for this antibody
Isotyp:
Rabbit IgG
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store the antibody at 4[o]C; stable for 6 months. For long-term storage; store at -20[o]C. Avoid repeated freeze and thaw cycles.
Hersteller:
Abeomics
Typ:
Antibodies:Polyclonal Antibody
  • Datenblatt des Herstellers:1.jpg
Fig:2 Immunohistochemical analysis of Cre in a formalin-fixed; paraffin embedded tissue section from the cerebellum of a Cre transgenic mouse using 20-1112 at 1:2000. In this mouse line; Cre is expressed in the brain; predominantly in the nuclei of most types of neurons. The arrow denotes an example of a stained neuron. Cre staining is also seen in the cytosol of cerebellar Purkinje cells. Hematoxylin-eosin counterstain.
Fig:3 Immunohistochemical analysis of Cre in a formalin-fixed; paraffin embedded tissue section from the cerebellum of a Cre transgenic mouse using 20-1112 at 1:2000. In this mouse line; Cre is expressed in the neurons brain. This mouse line also contains a LacZ reporter gene that becomes transcriptionally active upon Cre-mediated recombination. Brain tissue slices were stained with X-gal (blue color) prior to embedding. Cre staining is brown. Nuclear Red counterstain.
Fig:3 Immunohistochemical analysis of Cre in a formalin-fixed; paraffin embedded tissue section from the cerebellum of a Cre transgenic mouse using 20-1112 at 1:2000. In this mouse line; Cre is expressed in the neurons brain. This mouse line also contains a LacZ reporter gene that becomes transcriptionally active upon Cre-mediated recombination. Brain tissue slices were stained with X-gal (blue color) prior to embedding. Cre staining is brown. Nuclear Red counterstain.