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ACE2/VERO Stable Cell Line

Produktgrößen
1 vial
£2797,00
14-525ACL-1VIAL
Über dieses Produkt
SKU:
14-525ACL
Weitere Details:
ACE2/VERO Stable Cell Line is a stably transfected Vero E6 cell line which expresses human angiotensin-converting enzyme 2 (ACE2). ACE2 is a type I transmembrane metalloenzyme located on the outer surface of endothelial cells in the lung; arteries; heart; kidney and intestines. ACE2 cleaves the carboxyl-terminal amino acid phenylalanine from angiotensin II and hydrolyses it into the vasodilator angiotensin. ACE2 also serves as an entry receptor for some coronaviruses including HCoV-NL63; SARS-CoV and SARS-CoV-2 as the virus that causes COVID-19. Sequence data: hACE2 (accession number NP_068576) MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSS LASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQ NGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNE RLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYS RGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWT NLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLT DPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLL RNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTL PFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSN DYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPW TLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISL KSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFN FFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS IWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNT DDVQTSF
Versandbedingungen:
Dry Ice
Lagerbedingungen:
Immediately upon receipt; store in liquid nitrogen.
Hersteller:
Abeomics
Typ:
Cells:Cell Lines
Fig-2: Detection of ACE2 expression in ACE2/VERO cells by flow cytometry. ACE2/VERO cells were probed with biotinylated SARS-Cov-2 Spike RBD protein (Abeomics; Cat. No. 21-1005-B) at 1 x 10^5 cells per 1 µg protein on ice for 2 hours. Cells were washed with flow buffer and then incubated with PE-labeled Streptavidin on ice for 20 min. Cell were washed and then analyzed by flow cytometry.
Fig-3: Binding of biotinylated SARS-CoV-2 Spike RBD protein to human ACE2 in the ACE2/VERO stable cell line. ACE2/VERO cells were probed with different amounts of biotinylated SARS-CoV-2 Spike RBD protein (Abeomics; Cat. No. 21-1005-B) and analyzed by flow cytometry through fluorescent-labeled Streptavidin detection.
Fig-4: Genomic PCR analysis. Genomic DNAs were purified from ACE2/VERO stable cells and parental Vero E6 cells; and used as the PCR templates. PCR was performed using the target gene construct-specific primer sets; and the plasmid DNA of target gene construct was used as positive control.
Fig-4: Genomic PCR analysis. Genomic DNAs were purified from ACE2/VERO stable cells and parental Vero E6 cells; and used as the PCR templates. PCR was performed using the target gene construct-specific primer sets; and the plasmid DNA of target gene construct was used as positive control.
Fig-5: Cell Surface staining of human ACE2 in the ACE2/VERO stable cell line. ATTO 488-conjugated anti-hACE2 antibody (clone AC18F; Abeomics; Cat. No.: 10-10031-AT488) was used at 1µg/ 1x10^6 cells (Red). ATTO 488-conjugated mouse IgG1 was used as isotype control at 1µg/ 1x10^6 cells (Green).