Anti-Vaccinia Virus (Clone: VACV-301)-Purified No Carrier Protein
Produktgrößen
250µg
£583,00
12-8271-250UG
1 mg
£812,00
12-8271-1MG
Über dieses Produkt
- SKU:
- 12-8271
- translate.label.attr.clone:
- VACV-301
- Klonalität:
- Monoclonal
- Weitere Details:
- Specificity: VACV-301 activity is directed against the A27 antigen. Antigen Distribution: A27 is expressed on the mature virion particle surface. Background: Vaccinia virus (VACV) is a member of the Orthopoxvirus genus; which includes cowpox virus (CPXV); monkeypox virus (MPXV); and variola virus (VARV)1. Orthopoxviruses have a large and complex proteome; and various species share genetic and antigenic features. Indeed; an infection with one orthopoxvirus species; or vaccinia virus vaccination; protects against infection by other orthopoxviruses1;2;3. During infection; VACV has two antigenically distinct forms: mature virions (MV) or enveloped virions (EV). VACV-301 was generated from peripheral blood mononuclear cells (PMBC) obtained from donors who had survived MPXV infection or been immunized against vaccinia1. PMBCs were transformed with Epstein-Barr virus; screened by ELISA against orthopoxvirus antigens; and used to generate hybridomas. Hybridoma supernatants were tested for reactivity against each of 12 recombinant VACV antigens designated A21; A27; A28; A33; B5; D8; F9; J5; H2; H3; L1; and L5. A33 and B5 are surface antigens on EV; the rest are surface antigens on MV. Cross-reactivity against inactivated lysates of VACV-; CPXV-; and MPXV-infected cell monolayer cultures was also assessed. VACV-301 targets A27 on the MV particle surface and reacts to VACV antigen and lysate; CPXV lysate; MPXV antigen and lysate; and VARV antigen but not lysate1. In addition; VACV-301 binds to purified antigen from VACV; MPXV; and VARV as well as virus-infected cell lysates from VACV; CPXV; and MPXV to a lesser degree. Furthermore; VACV-301 strongly neutralizes VACV MV and MV plus complement (+C?); weakly neutralizes CPXV MV+C? but not MV alone; and neutralizes MPXV MV+C? more strongly than MV alone. When VACV-301 is mixed with other mAbs; neutralization and cross-neutralization are more efficient than in individual assays1. These mAb mixes also provide protection against VACV in mice. VACV-301 was also independently capable of preventing mortality and severe weight loss in C57BL/6 mice. VACV-301 is of the IgG3 isotype.
- Isotyp:
- Human IgG1Lambda
- Aufreinigung:
- Purity: >=90% monomer by analytical SEC and SDS-Page Preparation: Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are pu
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- This antibody may be stored sterile as received at 2-8[o]C for up to one month. For longer term storage; aseptically aliquot in working volumes without diluting and store at
- Hersteller:
- Abeomics
- Typ:
- Proteins, Peptides, Small Molecules & Other Biomolecules
- Datenblatt des Herstellers:download_pdf.php