Anti-Bundibugyo Ebolavirus; GP (Clone BDBV-317)-Purified No Carrier Protein
Produktgrößen
250µg
£583,00
12-8196-250UG
1 mg
£812,00
12-8196-1MG
Über dieses Produkt
- SKU:
- 12-8196
- Anwendung:
- Electron Microscopy
- translate.label.attr.clone:
- BDBV-317
- Klonalität:
- Monoclonal
- Weitere Details:
- Specificity: BDBV- 317 activity is directed against an epitope near the base of Bundibugyo ebolavirus (BDBV) glycoprotein (GP). BDBV-317 binds to the canonical heptad repeat 2 (HR2) domain (PDBID 5JQ3) near the membrane-proximal external region (MPER) of the GP; close to the viral membrane. Antigen Distribution: Ebola virus glycoprotein is a surface protein expressed on the virus envelope. Background: Ebola virus is a member of the Filoviridae family that causes severe disease in humans with a mortality rate of 25-90%1. Three Ebola species are responsible for lethal outbreaks: Zaire ebolavirus (EBOV); Bundibugyo ebolavirus (BDBV); and Sudan ebolavirus (SUDV). The Ebola virus envelope contains a single surface glycoprotein (GP) which is responsible for viral attachment to the host cell; endosomal entry; and membrane fusion1. GP is composed of two subunits; GP1 and GP2. GP1 has a heavily glycosylated mucin-like domain and a glycan cap. GP2 contains the internal fusion loop; transmembrane domain; and stalk. GP is the major target of neutralizing monoclonal antibody (mAb) and vaccine design against Ebola virus1;2;3. BDBV-317 is a GP mAb isolated from B cells of a survivor of the 2007 Uganda BDBV outbreak3. Peripheral blood mononuclear cells from the survivor were transformed with Epstein-Barr virus; CpG; and additional supplements. Subsequently; cell supernatants were screened by ELISA for binding to GPs from BDBV; EBOV; or MARV filoviruses. Positive cells were fused with HMMA2.5 myeloma cells by electrofusion and cloned by single-cell fluorescence-activated cell sorting. BDBV-317 binds to and neutralizes BDBV as well as EBOV heterologous Mayinga (historical) and Makona-Gueckedou-C07 (contemporary) strains3;4. BDBV-317 also neutralizes two strains of Reston virus and recognizes; but does not neutralize; SUDV4. BDBV-317 can also recognize a linear synthesized peptide spanning GP amino acids 620-635; suggesting a relatively linear epitope in vivo4. A single critical contact residue in GP2 (K633) is located in a region that spans the C-terminal part of HR2 and the N-terminal part of MPER4. Three BDBV-317 molecules bind simultaneously to the GP trimer4. BDBV-317 provides complete protection from death against EBOV (Mayinga strain) in mouse and partial protection in guinea pig4.
- Host:
- HEK293
- Isotyp:
- Human IgG1k
- Aufreinigung:
- Purity: >=90% monomer by analytical SEC and SDS-Page Preparation: Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are pu
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- This antibody may be stored sterile as received at 2-8[o]C for up to one month. For longer term storage; aseptically aliquot in working volumes without diluting and store at
- Hersteller:
- Abeomics
- Typ:
- Proteins, Peptides, Small Molecules & Other Biomolecules
- Datenblatt des Herstellers:download_pdf.php