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Proteine und Peptide

Anti-Dengue Virus (Clone: DENV-2D22)

Produktgrößen
100 µg
£649,00
12-8125-100UG
Über dieses Produkt
SKU:
12-8125
Zusätzliche Namen:
DENV
translate.label.attr.clone:
DENV-2D22
Klonalität:
Monoclonal
Weitere Details:
Reactivity Species : Dengue-Virus Expression Host : HEK-293 Endotoxin Level 1.0 EU/mg as determined by the LAL method Specificity : DENV-2D22 activity is DENV-2 specific and directed against the E homodimer at the DIII€¯+€¯glycan loop with serotype specificity on one E protein and DII around the fusion loop on the other E protein. Antibody clone DENV-2D22 was identified as strongly neutralizing; capable of inhibiting infection of DENV-2; and able to bind intact DENV-2 but not DIII or recombinant E. DENV-2D22 also did not bind to E protein by immunoblot. DENV-2D22 is E protein specific based on an escape mutant of DIII at R323G and recognizes a complex quaternary epitope displayed on the intact virus formed by DIII and DII on two different monomers within a single dimer. When the entire DENV-2 DIII region was inserted into the backbone sequence of a DENV-4 molecular clone to create a recombinant virus; DENV-2D22 was capable of binding and neutralization. Only five contact residues differ between DENV-2 and -4 in DIII; and these are likely critical for binding and neutralization. DENV-2D22 had no detectable ability to enhance DENV infection at a typical concentration range in a Ab-dependent enhancement assay; was capable of binding and neutralizing chimeric yellow fever-dengue vaccine virus serotype 2; and blocked viral infection of viremic blood for Ae. aegypti.
Host:
Human
Immunogen:
Sequenced from human survivors of who had experienced a DENV infection during travel to an endemic region.
Isotyp:
Human IgG1
Aufreinigung:
>=95% monomer by analytical SEC
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Functional grade preclinical antibodies may be stored sterile as received at 2-8[o]C for up to one year. For longer term storage; aseptically aliquot in working volumes without diluting and store at >= -70[o]C. Avoid Repeated Freeze Thaw Cycles.
Hersteller:
Abeomics
Typ:
Proteins, Peptides, Small Molecules & Other Biomolecules:Viral Particles