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Antikörper

Phospho-HS1 (Tyr397) (Clone: F12) rabbit mAb

Produktgrößen
20 µl
£631,00
12-4311-20UL
200 µl
£1021,00
12-4311-200UL
Über dieses Produkt
SKU:
12-4311
Zusätzliche Namen:
Hematopoietic lineage cell-specific protein; Hematopoietic cell-specific LYN substrate 1; LckBP1; p75; HCLS1
translate.label.attr.clone:
HS1Y397-F12
Klonalität:
Monoclonal
Konjugat:
Unconjugated
Weitere Details:
HS1 is expressed in lymphoid and hematopoietic cells; and is heavily post-translationally modified. HS1 deficient mouse models have demonstrated the protein's role in receptor-mediated apoptosis and proliferation. HS1 is phosphorylated at Tyr378 and Tyr397 by the kinase Syk; providing a high-affinity binding site for SH2 domains from the Src family. Following this interaction; HS1 is then phosphorylated at Tyr222 by c-Fgr; Lyn; and Fyn kinases. HS1 plays an important role in T cell signaling; where HS1 phosphorylation recruits and activates Vav1 at the immune synapse. As a homolog of the actin binding protein cortactin; HS1 has been shown to mediate neutrophil chemotaxis through phosphorylation of tyrosines 222; 378; and 397.
Format:
Purified
Host:
Rabbit
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Tyr397 of human phospho HS1
Isotyp:
Rabbit IgG1k
Reaktivitäten:
Human, Mouse
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at -20[o]C. Avoid repeated freeze and thaw cycles.
Hersteller:
Abeomics
Typ:
Antibodies:Monoclonal Antibody
  • Datenblatt des Herstellers:1.jpg
Fig 2 : Western blot analysis of Ramos cell extract; untreated or treated with 300 nM Thapsigargin for 30 min using HS1 (Tyr397) antibody HS1Y397-F12 at 0.01 µg/mL.
Fig-3: Peptide blocking flow cytometric analysis of Ramos cells secondary antibody only negative control (light blue) or untreated (red) or treated with pervandadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-HS1 (Tyr397) antibody HS1Y397-F12 at 0.01µg/mL.
Fig-4: Western blot analysis of Ramos cell extract untreated or treated with 300 nM thapsigargin for 30 min using 10 ng/mL Phospho-HS1 (Tyr397) antibody HS1Y397-F12 at 0.01µg/mL. Company C antibody at 3 ng/mL (manufacturer's recommended concentration) developed using the same exposure.
Fig-4: Western blot analysis of Ramos cell extract untreated or treated with 300 nM thapsigargin for 30 min using 10 ng/mL Phospho-HS1 (Tyr397) antibody HS1Y397-F12 at 0.01µg/mL. Company C antibody at 3 ng/mL (manufacturer's recommended concentration) developed using the same exposure.
Fig-5: Flow cytometric analysis of mouse cells secondary antibody only negative control (blue) or control (red) or stimulated (green) using Phospho-HS1 (Tyr397) antibody HS1Y397-F12 at 0.01µg/mL.